Browse Items (11810 total)
Sort by:
-
Anthocyanin Production from Plant Cell and Organ Cultures In Vitro
Anthocyanins are water-soluble pigments found in plants. They exist in various colors, including red, purple, and blue, and are utilized as natural colorants in the food and cosmetics industries. The pharmaceutical industry uses anthocyanins as therapeutic compounds because they have several medicinal qualities, including anti-obesity, anti-cancer, antidiabetic, neuroprotective, and cardioprotective effects. Anthocyanins are conventionally procured from colored fruits and vegetables and are utilized in the food, pharmaceutical, and cosmetic industries. However, the composition and concentration of anthocyanins from natural sources vary quantitively and qualitatively; therefore, plant cell and organ cultures have been explored for many decades to understand the production of these valuable compounds. A great deal of research has been carried out on plant cell cultures using varied methods, such as the selection of suitable cell lines, medium optimization, optimization culture conditions, precursor feeding, and elicitation for the production of anthocyanin pigments. In addition, metabolic engineering technologies have been applied for the hyperaccumulation of these compounds in varied plants, including tobacco and arabidopsis. In this review, we describe various strategies applied in plant cell and organ cultures for the production of anthocyanins. 2023 by the authors. -
Light as an elicitor for enhanced production of secondary metabolites in plant cell, tissue, and organ cultures
Currently, in vitro cell, tissue, and organ cultures are used to produce plant secondary metabolites that are used as natural coloring agents, nutraceuticals, and medications. Various strategies have been applied for the hyperaccumulation of biomass and bioactive secondary compounds in vitro. The elicitation of cultured cells and organs with biotic and abiotic elicitors is an excellent strategy that has yielded promising results. Among various abiotic elicitors, light parameters such as light quality, intensity, and photoperiod have evolved as biotechnological tools to elicit cultures. Of the various light sources tested, ultraviolet (UV) lights, particularly UV-B, red, blue, and a mixture of light emitted by fluorescent light or light-emitting diodes, have yielded outstanding results and boosted the accumulation of bioactive compounds in cultured cells and organs. The objective of the current study was to evaluate light as an elicitor source and summarize the advantages and limitations of various light sources as elicitors for the bioaccumulation of secondary metabolites in vitro. The mechanism of the elicitation of secondary metabolism by UV and spectral light is discussed in this review. The Author(s), under exclusive licence to Springer Nature B.V. 2024. -
Nanomaterials as novel elicitors of pharmacologically active plant specialized metabolites in cell and organ cultures: current status and future outlooks
Specialized plant metabolites, such as phenolics, terpenes, terpenoids, nitrogen-containing compounds, and sulfur-containing compounds, are commercially valuable owing to their wide array of applications in the medical, pharmacological, cosmetic, agriculture, and food industries. Procuring valuable specialized metabolites from wild or cultivated plants is desirable; however, the concentrations and quality of secondary compounds vary between samples. Therefore, plant cells and organ cultures have been selected as viable alternatives for producing specialized metabolites. Elicitation is a strategy used to enhance the accumulation of specialized compounds in cell and organ cultures. Different biotic substances, including signaling chemicals such as salicylic acid and methyl jasmonate, elements of plant cell walls (cellulose and pectin), polysaccharides from microbes (chitin and glucan), and abiotic substances such as inorganic salts, heavy metals, UV radiation, and high salinity, have been successfully tested and used as elicitors for the hyperaccumulation of bioactive substances in cell and organ cultures. Recently, metals, metal oxide nanoparticles, and carbon-based nanomaterials have been used as unique elicitors to boost the synthesis of bioactive compounds in cell and organ cultures. The applications and usage of nanoparticles as elicitors in plant cell and organ cultures are summarized in this review. The mechanism of elicitation, toxicity, benefits, and drawbacks of using nanoparticles in plant cell and organ cultures are discussed. Graphical abstract: (Figure presented.) The Author(s), under exclusive licence to Springer Nature B.V. 2023. -
Production of specialized metabolites in plant cell and organo-cultures: the role of gamma radiation in eliciting secondary metabolism
Purpose: To provide an updated summary of recent advances in the application of gamma irradiation to elicit secondary metabolism and for induction of mutations in plant cell and organ cultures for the production of industrially important specialized metabolites (SMs). Conclusions: Research on the application of gamma radiation with plants has contributed a lot to microbial decontamination of seeds, and the promotion of physiological processes such as seed germination, seedling vigor, plant growth, and development. Various studies have demonstrated the influence of gamma rays on the morphology, physiology, and biochemistry of plants. Recent research efforts have also shown that low-dose gamma (5100 Gy) irradiation can be utilized as an expedient solution to alleviate the deleterious effect of abiotic stresses and to obtain better yields of plants. Inducing mutagenesis using gamma irradiation has also evolved as a better option for inducing genetic variability in crops, vegetables, medicinal and ornamentals for their genetic improvement. Plant SMs are gaining increasing importance as pharmaceutical, therapeutic, cosmetic, and agricultural products. Plant cell, tissue, and organ cultures represent an attractive alternative to conventional methods of procuring useful SMs. Among the varied approaches the elicitor-induced in vitro culture techniques are considered an efficient tool for studying and improving the production of SMs. This review focuses on the utilization of low-dose gamma irradiation in the production of high-value SMs such as phenolics, terpenoids, and alkaloids. Furthermore, we present varied successful examples of gamma-ray-induced mutations in the production of SMs. Copyright 2024 Taylor & Francis Group LLC. -
Production of biomass and bioactive compounds from cell and organ cultures of ginseng, He-shou-wu, purple coneflower, and St. John's wort for the use in cosmetic industry
Plants and their products have been utilized as raw materials in the preparation of cosmetics for millennia. Currently, research is being done to find new plant materials that may be used as ingredients in cosmetic preparations, including body sprays, shampoos, conditioners, hair dyes/sprays, and other cosmetics. The plants that are used in the preparation of cosmetic products are usually procured from nature, however, the quality and quantity of bioactive ingredients present in the biomass vary based on the species, environment, and geographical locations from where the material has been procured. In addition, procurement of plant material from natural resources may lead to a shortage of material and even endanger the status of rare plants in the natural environment. Therefore, there is increased interest in the use of plant cell, tissue, and organ cultures (PCTOC) for the production of raw materials and bioactive specialized metabolites. There is also scope for increasing the accumulation of biomass and bioactive compounds in PCTOC by adopting various strategies such as optimization of culture medium, culture environment, elicitation, and other bioprocess methods. Furthermore, PCTOC-produced raw materials are free from contaminants, pesticides, and heavy metals and an important benefit of producing biomass in vitro is that it is easily accepted by regulatory authorities and consumers. In the current review, we describe the bioactive compounds of ginseng, purple coneflower, He-shou-wu, and St. John's wort which have cosmetological importance. Additionally, we elucidate the PCTOC method adopted for the production of biomass and bioactive compounds in these plants. 2023 SAAB -
Bioreactor configurations for adventitious root culture: recent advances toward the commercial production of specialized metabolites
In vitro plant cell and organ cultures are appealing alternatives to traditional methods of producing valuable specialized metabolites for use as: pharmaceuticals, food additives, cosmetics, perfumes, and agricultural chemicals. Cell cultures have been adopted for the production of specialized metabolites in certain plants. However, in certain other systems, adventitious roots are superior to cell suspension cultures as they are organized structures that accumulate high levels of specialized metabolites. The cultivation of adventitious roots has been investigated in various bioreactor systems, including: mechanically agitated, pneumatically agitated, and modified bioreactors. The main relevance and importance of this work are to develop a long-lasting industrial biotechnological technology as well as to improve the synthesis of these metabolites from the plant in vitro systems. These challenges are exacerbated by: the peculiarities of plant cell metabolism, the complexity of specialized metabolite pathways, the proper selection of bioreactor systems, and bioprocess optimization. This reviews major objective is to analyze several bioreactor types for the development of adventitious roots, as well as the advantages and disadvantages of each type of bioreactor, and to describe the strategies used to increase the synthesis of specialized metabolites. This review also emphasizes current advancements in the field, and successful instances of scaled-up cultures and the generation of specialized metabolites for commercial purposes are also covered. 2023 Informa UK Limited, trading as Taylor & Francis Group. -
Bioreactor systems for micropropagation of plants: present scenario and future prospects
Plant micropropagation has been adapted in the fields of agriculture, horticulture, forestry, and other related fields for large-scale production of elite plants. The use of liquid media and adoption of bioreactors have escalated the production of healthy plants. Several liquid-phase, gas-phase, temporary immersion, and other modified bioreactors have been used for plant propagation. The design, principle, operational mode, merits, and demerits of various bioreactors used for the regeneration of propagules, such as bulblets, cormlets, rhizomes, microtubers, shoots (subsequent rooting), and somatic embryos, are discussed here. In addition, various parameters that affect plant regeneration are discussed with suitable examples. Copyright 2023 Murthy, Joseph, Paek and Park. -
Anthraquinone Production from Cell and Organ Cultures of Rubia Species: An Overview
The Rubia genus includes major groups of medicinal plants such as Rubia cordifolia, Rubia tinctorum, and Rubia akane. They contain anthraquinones (AQs), particularly alizarin and purpurin, which have pharmacological effects that are anti-inflammatory, antioxidant, anticancer, hemostatic, antibacterial, and more. Alizarin and purpurin have been utilized as natural dyes for cotton, silk, and wool fabrics since the dawn of time. These substances have been used in the cosmetics and food industries to color products. The amount of AQs in different Rubia species is minimal. In order to produce these compounds, researchers have established cell and organ cultures. Investigations have been conducted into numerous chemical and physical parameters that affect the biomass and accumulation of secondary metabolites in a cell, callus, hairy root, and adventitious root suspension cultures. This article offers numerous techniques and approaches used to produce biomass and secondary metabolites from the Rubia species. Additionally, it has been emphasized that cells can be grown in bioreactor cultures to produce AQs. 2022 by the authors. -
Production of anthraquinones from cell and organ cultures of Morinda species
Abstract: Since ancient times, Morinda species, particularly Morinda citrifolia, have been used for their therapeutic benefits. Iridoids, anthraquinones, coumarins, flavonoids, lignans, phytosterols, and carotenoids are examples of natural substances with bioactivity. Anthraquinone derivatives are the most significant of these chemicals since they are utilized as natural coloring agents and have a wide range of medicinal functions. Utilizing cell and organ cultures of Morinda species, various biotechnological methods have been developed for the bioproduction of anthraquinone derivatives. The generation of anthraquinone derivatives in cell and organ cultures is summarized in this article. The methods used to produce these chemicals in bioreactor cultures have also been examined. Key points: This review investigates the potential of cell and organ cultures for anthraquinone synthesis. The overproduction of anthraquinones has been addressed using a variety of techniques. The use of bioreactor technologies for anthraquinone manufacturing is highlighted. 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. -
Nutritional Value, Fatty Acid and Phytochemical Composition, and Antioxidant Properties of Mysore Fig (Ficus drupacea Thunb.) Fruits
Ficus drupacea is a fruit-bearing tree that is distributed in Southeast Asia and Australia. The objective of this research was to ascertain the following with regard to ripened fruits: (i) their nutritional value, (ii) their mineral status, (iii) the fatty acid composition of fruit and seed oil, (iv) their phytochemical makeup, and (v) their antioxidant properties. The ripened fruits contained 3.21%, 3.25%, 0.92%, 1.47%, and 2.20% carbohydrate, protein, fat, ash, and fiber, respectively. Fruits had an energy content of 30.18 kcal/100 g. In terms of mineral content, the fruit was rich in potassium, magnesium, calcium, and nitrogen, with values of 21.03, 13.24, 11.07, and 4.13 mg/g DW. Iron, zinc, manganese, and boron had values of 686.67, 124.33, 114.40, and 35.78 g/g DW, respectively. The contents of oxalate and phytate were 14.44 and 2.8 mg/g FW, respectively. The fruit and seed oil content were 0.67 and 8.07%, respectively, and the oils physicochemical properties were comparable to those of fig fruit and seed oils. Omega-3 (?-linolenic acid), omega-6 (linoleic acid), and omega-9 (oleic acid) fatty acids were abundant in the oils. Fruit extracts in acetone, methanol, and water have greater concentrations of phenolics, flavonoids, and alkaloids. The 2,2-diphenyl-2-picrylhydrazyl, total antioxidant activity, and ferric reducing antioxidant power assays demonstrated increased antioxidant activities in close correlation with the higher concentrations of phenolics, flavonoids, and alkaloids. The results of this study demonstrate that the fruits of F. drupacea are a strong source of nutrients and phytochemicals, and they merit more investigation and thought for possible uses. 2024 by the authors. -
JUDE: An Ultraviolet Imaging Telescope pipeline
The Ultraviolet Imaging Telescope (UVIT) was launched as part of the multi-wavelength Indian AstroSat mission on 28 September, 2015 into a low Earth orbit. A 6-month performance verification (PV) phase ended in March 2016, and the instrument is now in the general observing phase. UVIT operates in three channels: visible, near-ultraviolet (NUV) and far-ultraviolet (FUV), each with a choice of broad and narrow band filters, and has NUV and FUV gratings for low-resolution spectroscopy. We have written a software package (JUDE) to convert the Level 1 data from UVIT into scientifically useful photon lists and images. The routines are written in the GNU Data Language (GDL) and are compatible with the IDL software package. We use these programs in our own scientific work, and will continue to update the programs as we gain better understanding of the UVIT instrument and its performance. We have released JUDE under an Apache License. 2017 Elsevier B.V. -
Fusion of medical image using STSVD
The process of uniting medical images which are taken from different types of images to make them as one image is a Medical Image Fusion. This is performed to increase the image information content and also to reduce the randomness and redundancy which is used for clinical applicability. In this paper a new method called Shearlet Transform (ST) is applied on image by using the Singular Value Decomposition (SVD) to improve the information content of the images. Here two different images Positron Emission Tomography (PET) and Magnetic Resonance Imaging (MRI) are taken for fusing. Initially the ST is applied on the two input images, then for low frequency coefficients the SVD method is applied for fusing purpose and for high frequency coefficients different method is applied. Then fuse the low and high frequency coefficients. Then the Inverse Shearlet Transform (IST) is applied to rebuild the fused image. To carry out the experiments three benchmark images are used and are compared with the progressive techniques. The results show that the proposed method exceeds many progressive techniques. Springer Nature Singapore Pte Ltd. 2017. -
Progress in psycho-oncology with special reference to developing countries
Purpose of reviewPsycho-oncology has completed 25 years. There is growing recognition of the psychosocial needs of persons living with cancer and the role of sociocultural factors in addressing the needs. This review addresses the research in developing countries relating to distress associated with living with cancer and psychosocial care.Recent findingsThere is growing recognition of the emotional needs, understanding of the sociocultural aspects of the emotional responses of persons, caregivers, role of resilience and posttraumatic growth and spirituality in cancer care. Psychosocial aspects of cancer are largely influenced by social, economic, cultural, religious and health systems. A number of innovative approaches to care like use of yoga, financial and material support and involvement of caregivers have been implemented. A positive development is the increasing professional attention to document and develop innovative care programmes.SummaryA significant proportion of the general population are living with cancer. There are significant psychosocial needs largely influenced by social, economic, cultural, religious aspects of the communities. There are a wide range of interventions from self-care to professional care to address the needs. In developing countries, there is need for longitudinal studies of psycho-social experiences, develop interventions that are culturally appropriate, along with enhanced use of information technology along with evaluation of interventions. 2019 Wolters Kluwer Health, Inc. All rights reserved. -
Effect of temperature modulation on Rayleigh-Benard convection in a rotating layer of a ferromagnetic fluid
The stability of a rotating horizontal layer of ferromagnetic fluid heated from below is examined when, in addition to a steady temperature difference between the walls of the layer, a time-dependent sinusoidal perturbation is applied to the wall temperatures. Only infinitesimal disturbances are considered. The effects of the oscillating temperature field are treated by a perturbation expansion in powers of the amplitude of the applied field. The onset criterion is derived when the condition for the principle of exchange of stabilities is valid. The shift in the critical Rayleigh number is calculated as a function of the frequency of modulation, magnetic parameters, Taylor number and Prandtl number. The effect of various parameters is found to be significant for moderate values of the frequency of modulation. It is shown that, when the thermal excitation is symmetric, supercritical motion is more pronounced for low Prandtl number ferro fluids. Further, for the case in which only the bottom wall temperature is modulated, the effect of rotation is to stabilize the system at low frequencies and the opposite is true for moderately large frequencies. The problem throws light on external means of controlling convection in ferromagnetic fluid applications. -
Prob(E)abilities for enhanced research and interdisciplinarity: An exploration of innovative practices in english studies, languages, and media
An analysis of the history of research as well as contemporary trends in higher education in India reveals the predominance of scientific research. Public policies, developmental strategies, and market forces often determine the nature and output of research in humanities and social sciences. Research in literatures, languages, and media needs articulationa process that reveals the significant interdisciplinary interventions that can be brought to the process of research and highlights the need for purpose-driven research as research rather than need-based research for research. This paper offers a critical overview of the role of research in universities in India, contemporary approaches to research at the national level, and the need to engage in interdisciplinary, collaborative, and action-based research, especially in humanities and social sciences departments in universities across India. Further, the paper offers a few examples of interdisciplinary and collaborative research to highlight the need for a symbiotic approach to academic research. 2020 IUP. All Rights Reserved. -
Amberlite-15 promoted an unprecedented aza Michael rearrangement for one pot synthesis of dihydroquinazolinone compounds
A new one pot multicomponent annulation strategy for the synthesis of various dihydroquinazolinone compounds has been developed using Amberlite-15 as a catalyst, giving good to moderate yields. In this reaction the substrate scope for amines and aldehydes was also investigated. The reaction has been checked on a large scale and the possible reaction mechanism has also been proposed. The Royal Society of Chemistry 2018. -
Vappav /
Patent Number: 202241028305, Applicant: Sashi Kumar D.
The primary objective and the motto behind this invention of the device Vappav is for the safety of food items and food parcels which needs to have a minimum contact from people and to make sure that the delivery of these parcels are safe without many people touching it, which further ensures the safety and hygienic of the food items. -
Is Bitcoin a Safe Haven for Indian Investors? A GARCH Volatility Analysis
This paper attempts to understand the dynamic interrelationships and financial asset capabilities of Bitcoin by analysing several aspects of its volatility vis-a-vis other asset classes. This study aims to analyse the volatility dynamics of the returns of Bitcoin. An asymmetric GARCH model (EGARCH) is used to investigate whether Bitcoin may be useful in risk management and ideal for risk-averse investors in anticipation of negative shocks to the market (leverage effect). This paper also examines Bitcoin as an investment and hedge alternative to gold as well as NSE NIFTY using a multivariate DCC GARCH model. DCC GARCH models are also used to check whether correlation (co-movement) between the markets is time-varying, examine returns and volatility spillovers between markets and the effect of the outbreak of COVID-19 in India on the investigated markets. The results show that given the supply of Bitcoin is fixed, low returns realisation is equivalent to excess supply over demand wherein investors are selling off Bitcoin during bad times. The positive co-movement between Bitcoin and gold during the COVID-19 outbreak shows that investors perceived Bitcoin as a relatively safe investment. However, overall analysis shows that Bitcoin was not considered a safe hedge and an investment option by Indian investors during the study period. 2022 by the authors. -
Wireless Communication for Robotic Process Automation Using Machine Learning Technique
Machine intelligence is what has been generated by programming computers with certain aspects of human intellect, like training, solving problems, and priority setting. A machine can solve a number of complicated issues using these capabilities. In major industries, such as customer support and manufacturing, machine intelligence is now being employed. The growth and quick development of digital technology and artificial intelligence (AI) technologies are becoming more and more difficult. At now, sophisticated manufacturing, the world of invention, and broad acceptance are undergoing a fast transition. Robotics is much more vital as it may now be related to the human brain by the connection between machine and brain, as AI develops. The world's economy faces substantial difficulties by increasing productivity in the manufacturing industry. This study examines the present progress of robotic communication styles of artificial intelligence (AI). In many specific applications, communication between members of a robotic group or even people becomes vital. The paper solves the problem of implementation of an independent industry mobile robot in all fields in the major business, live interactive, planning, mobile robot technologies, and intending. In order to identify the best solution to this issue, a mixed integer robotic model has been developed. 2022 C. Murugamani et al. -
Machine Learning and Signal Processing Methodologies to Diagnose Human Knee Joint Disorders: A Computational Analysis
Computer-aid diagnostic (CAD) has emerged as a highly innovative research topic in diverse fields which includes medical imaging systems, radiology diagnostics, and so on. These are the systems that majorly assist doctors by the way of interpretation of medical data or images. In the diagnosis of knee joint disorder technique, both time and frequency-based analysis can be done. These non-stationary and non-linear signals are processed into three important methods, namely VMD, TVF-EMD, and CEEMDAN. To analyze the vibroarthrographic (VAG) signal, the initial stage is to compute the mode strategies termed as intrinsic mode functions (IMFs) which can be attained only after performing the transformations. In our chapter, we analyzed Complete Ensemble Empirical Mode Decomposition with Adaptive Noise (CEEMDAN) for computing the mode signals. The CEEMDAN method utilized the time and frequency data for the available features. The feature extraction depends purely on pixel intensity and the statistical parameters. The classification of available data samples is done through the Least Square Support Vector Machine (LS-SVM) and SVM-Recursion of Feature Elimination (SVM-RFE) for the efficient analysis of healthy and unhealthy data samples. 2024 selection and editorial matter, Hemachandran K., Raul V. Rodriguez, Umashankar Subramaniam, and Valentina Emilia Balas; individual chapters, the contributors.