Production Of Boeravinone B And Related Bioactive Molecules From Cell Cultures Of Punarnava, Boerhavia Diffusa Linn.

Title

Production Of Boeravinone B And Related Bioactive Molecules From Cell Cultures Of Punarnava, Boerhavia Diffusa Linn.

Creator

Wudali, Narasimha Sudheer

Contributor

N,Praveen

Description

Boerhavia diffusa L., commonly referred to as punarnava, belongs to the Nyctaginaceae family. It contains a variety of phytochemicals that contribute to the plant's pharmacological activities. Among these compounds, Boeravinone-B, a significant secondary metabolite synthesized within the plant, has been reported to possess various pharmacological properties, including antioxidant, anti-inflammatory, cardio-protective, antimicrobial, and anti-cancer activities. Given its potential, there is a growing need to produce Boeravinone-B through biotechnological approaches such as plant tissue cultures. However, limited studies have been conducted on its production via tissue culture. Therefore, our objective was to produce Boeravinone-B using callus and cell suspension cultures. To induce callus, different auxins (2,4-D, NAA, IAA, IBA, and picloram) and cytokinins (kinetin, BAP, TDZ, and 2-iP) alone and in combination to maximize biomass and Boeravinone-B production was employed. Results showed that semi-solid medium supplemented with 5.0 mg/L 2,4-D produced the highest biomass and Boeravinone-B production from leaf explants. Subsequently, we established cell suspension cultures and observed that a combination of 2.0 mg/L picloram and 5.0 mg/L NAA yielded the highest production of Boeravinone-B. We further optimized cultural conditions such as inoculum density, carbon sources, sucrose concentration, MS medium strength, pH, ammonium and nitrate ratio, and various concentrations of macronutrients to enhance Boeravinone-B production. Additionally, we explored elicitation strategies using both biotic and abiotic components to determine their effects on Boeravinone-B production. Elicitation involved the use of polysaccharides (chitosan), fungi (yeast extract, Aspergillus niger and Cordyceps militaris) bacteria (Escherichia coli and Bacillus subtilis), and algal members (Valonia utricularis and Spirulina platensis). Likewise, signalling molecules (salicylic acid, sodium nitroprusside, and ethephon), polyamines (putrescine, spermine, and spermidine), and heavy metal salts (lead acetate, cadmium chloride, and lanthanum chloride) to stimulate Boeravinone-B production. Significant variations were observed among the elicitors in their ability to promote Boeravinone-B production, with the order of effectiveness being spermidine > Spirulina platensis > salicylic acid > spermine > sodium nitroprusside > Aspergillus niger > Valonia utricularis > Cordyceps militaris > yeast extract > putrescine. By employing these elicitation strategies alongside advancements in molecular aspects and large-scale production in bioreactors, we can ensure a sustainable supply of Boeravinone-B and its associated metabolites.

Source

Author's Submission

Date

2024-01-01

Publisher

Christ(Deemed to be University)

Subject

Life Sciences

Rights

Open Access

Relation

61000303

Format

PDF

Language

English

Type

PhD

Identifier

http://hdl.handle.net/10603/547681