Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource PHD PhD PhD Thesis Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Relation A related resource 61000310 Title A name given to the resource Clonning and Characterization of An Exported Protein Present in the RD7 Region of Clinical Isolates of Mycobacterium Tuberculosis Subject The topic of the resource Life Sciences Description An account of the resource The bacterium Mycobacterium tuberculosis is responsible for causing the disease newlinetuberculosis in mammals, which is regarded as one of the oldest diseases haunting the human race. The only available tuberculosis vaccine Bacillus Calmette-Guerine (BCG), is effective against childhood tuberculosis but is regarded as having low efficacy in conferring protection in the case of tuberculosis in adults. A comparison of the M. tuberculosis H37Rv strain and clinical isolates from Kerala had earlier revealed that the clinical strains have a distinctive 4.5 kb genomic sequence that is lacking from the H37Rv strain in the RD7 region. The RD7 is a distinctive genomic region that is absent in M. tuberculosis H37Rv and Mycobacterium bovis BCG strain. The 4.5 kb genomic sequence is projected to include 6 potential ORFs by newlineNCBI ORF prediction tool, one of which Novel Hypothetical Protein (NHP2) is anticipated to encode an exported protein with a length of 268 amino acids. Studies demonstrate that Mycobacterium tuberculosis secretory proteins such as the Ag85 complex, the ESAT-6 family protein, and the PE-PPE family proteins were newlineeffective vaccine candidates because they trigger T cells. Here, we present an indepth analysis of the exported protein, which is 268 amino acids long. The putative exported protein with a gene 807 bp long was PCR amplified and cloned in the expression vector pET-32a for expression. The protein was over expressed using Isopropyl D-1-thiogalactopyranoside (IPTG) and was isolated and purified using column chromatography. Bioinformatics studies were conducted to study the characteristics of the expressed protein. A novel putative mycobacterial protein discovered by subtractive hybridization was studied for its potential as a vaccine candidate using cutting-edge computer technologies. Creator An entity primarily responsible for making the resource Kaviya, P K Source A related resource from which the described resource is derived Author's Submission Publisher An entity responsible for making the resource available Christ(Deemed to be University) Date A point or period of time associated with an event in the lifecycle of the resource 2024-01-01 Contributor An entity responsible for making contributions to the resource S, Suma. Rights Information about rights held in and over the resource Open Access Format The file format, physical medium, or dimensions of the resource PDF Language A language of the resource English Type The nature or genre of the resource PhD Identifier An unambiguous reference to the resource within a given context <a href="http://hdl.handle.net/10603/551690" target="_blank" rel="noreferrer noopener">http://hdl.handle.net/10603/551690</a>