Process Development for Mass Production of Cordycepin Using Fermentation Technology

Title

Process Development for Mass Production of Cordycepin Using Fermentation Technology

Creator

Kondapalli, Vamsi Krishna

Contributor

Alok, Malaviya

Description

Cordyceps is a rare and exotic medicinal genus that has been utilised for generations in traditional Chinese medicine. China, Bhutan, Nepal, the Tibetan Plateau, and the newlinenortheastern parts of India are the main areas where they may be noticed. Cordycepin newline(C10H13O3N5), an adenosine derivative generated naturally by Cordyceps militaris, has essential pharmacological effects. Cordycepin has been shown to have anti-tumor/antiproliferative, anti-metastatic, apoptosis-inducing, anti-malaria, anti-microbial, antifungal, anti-diabetic/hypoglycemic, and anti-inflammatory qualities, among other things. The scarcity of native Cordyceps spp., as well as illicit commerce and a lack of growing methods in natural habitats, limit the supply of this prized fungus for therapeutic applications. As a result, an attempt was made to standardize the technique for large-scale cordycepin manufacturing in a laboratory setting. To measure the cordycepin, analytical methods by using High performance liquid chromatography (HPLC) and Ultraviolet Visible spectroscopy (UV Spec.) were established. Furthermore, different methods were optimised and established to successfully extract cordycepin from biomass. Solid state fermentation (SSF) was performed to grow the fruiting bodies of C. militaris in jars. A novel technology was developed using cotton in the SSF which improved the cordycepin content by 138.42 %. Different liquid culture methods (static, submerged, and mix) were also studied; the Static mode was chosen for future media optimization studies. Initially, different parameters like the effect of pH, light, media volume, and inoculum percentage were optimised that affect the cordycepin production. Further, numerous media components, including carbon newlinesources, nitrogen sources, metals, salts and also the impact of vitamins, amino acids, newlineand adenosine that can influence the cordycepin production were optimized employing the One Factor at a time (OFAT) method.

Source

Author's Submission

Date

2024-01-01

Publisher

Christ(Deemed to be University)

Subject

Life Sciences

Rights

Open Access

Relation

61000423

Format

PDF

Language

English

Type

PhD

Identifier

http://hdl.handle.net/10603/620498