Wound Healing, Cell Viability and Antimicrobial Potency of Mucus from Pangasianodon hypophthalmus

Title

Wound Healing, Cell Viability and Antimicrobial Potency of Mucus from Pangasianodon hypophthalmus

Creator

Gomathy M.; Pappuswamy M.

Description

Acute and chronic wounds are the major cause of death according to World Health Organization (WHO), in which, antimicrobial resistance is considered to be a major plight. In this regard, our study is aimed at developing an antimicrobial agent using the mucus of Pangasianodon hypophthalmus against the clinically resistant microbial pathogens and to evaluate the cell cytotoxicity and cell viability followed by an in vitro wound healing analysis. The evaluation of antimicrobial activity was performed through well diffusion method and micro dilution method. The cell cytotoxicity and cell viability were assessed using MTT assay. The cell migration and in vitro wound healing was performed using scratch assay. The acidic extracts of mucus showed antimicrobial activity against the eight different selected bacterial strains while the organic extract showed against seven bacterial strains. L929 showed a cell viability of 102.96% at a concentration of 75 g/mL and did not show cell toxicity effect up to the concentration of 300 g/mL. In the in vitro wound healing analysis, the cell migration rate was 99.27% in the treated cells while, the untreated showed only 94.68%. The current research work clearly shows that the mucus of P. hypophthalmus possesses antimicrobial activity and wound healing potency. Furthermore, gene expression analysis and in vivo trials have to be performed for a thorough understanding of the actual cellular mechanism of wound healing. The Author(s) 2024.

Source

Journal of Pure and Applied Microbiology, Vol-18, No. 4, pp. 2411-2423.

Date

2024-01-01

Publisher

Journal of Pure and Applied Microbiology

Subject

Cell Cytotoxicity; Cell Migration; Mucus Extracts; Pangasianodon hypophthalmus; Wound Healing

Coverage

Gomathy M., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Pappuswamy M., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India

Rights

All Open Access; Gold Open Access

Relation

ISSN: 9737510

Format

Online

Language

English

Type

Article

Identifier

https://doi.org/10.22207/JPAM.18.4.14

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