Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Assessing anticancer properties of PEGylated platinum nanoparticles on human breast cancer cell lines using in-vitro assays Subject The topic of the resource anticancer property; AO/PI dual staining; DCFH DA ROS assay; MTT assay; platinum nanoparticles; polyethylene glycol Description An account of the resource This study describes the in-vitro cytotoxic effects of PEG-400 (Polyethylene glycol-400)-capped platinum nanoparticles (PEGylated Pt NPs) on both normal and cancer cell lines. Structural characterization was carried out using x-ray diffraction and Raman spectroscopy with an average crystallite size 5.7 nm, and morphological assessment using Scanning electron microscopy (SEM) revealed the presence of spherical platinum nanoparticles. The results of energy-dispersive x-ray spectroscopy (EDX) showed a higher percentage fraction of platinum content by weight, along with carbon and oxygen, which are expected from the capping agent, confirming the purity of the platinum sample. The dynamic light scattering experiment revealed an average hydrodynamic diameter of 353.6 nm for the PEGylated Pt NPs. The cytotoxicity profile of PEGylated Pt NPs was assessed on a normal cell line (L929) and a breast cancer cell line (MCF-7) using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The results revealed an IC50 of 79.18 ?g ml?1 on the cancer cell line and non-toxic behaviour on the normal cell line. In the dual staining apoptosis assay, it was observed that the mortality of cells cultured in conjunction with platinum nanoparticles intensified and the proliferative activity of MCF-7 cells gradually diminished over time in correlation with the increasing concentration of the PEGylated Pt NPs sample. The in vitro DCFH-DA assay for oxidative stress assessment in nanoparticle-treated cells unveiled the mechanistic background of the anticancer activity of PEGylated platinum nanoparticles as ROS-assisted mitochondrial dysfunction. 2024 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved. Creator An entity primarily responsible for making the resource Aswathy F.A.W.; Jose J.; Anila A.E.I. Source A related resource from which the described resource is derived Biomedical Physics and Engineering Express, Vol-10, No. 6 Publisher An entity responsible for making the resource available Institute of Physics Date A point or period of time associated with an event in the lifecycle of the resource 2024-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1088/2057-1976/ad795d" target="_blank" rel="noreferrer noopener">https://doi.org/10.1088/2057-1976/ad795d</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85204819867&amp;doi=10.1088%2F2057-1976%2Fad795d&amp;partnerID=40&amp;md5=9bb20fdd8e84ea8f44568762e4f0f380" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85204819867&amp;doi=10.1088%2f2057-1976%2fad795d&amp;partnerID=40&amp;md5=9bb20fdd8e84ea8f44568762e4f0f380</a> Rights Information about rights held in and over the resource Restricted Access Relation A related resource ISSN: 20571976; PubMed ID: 39260382 Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Aswathy F.A.W., Department of Physics and Electronics, Christ University, Karnataka, Bengaluru, 560029, India; Jose J., Division of Microbiology, Department of Biosciences, Rajagiri College of Social Sciences (Autonomous), Kerala, Cochin, 683104, India; Anila A.E.I., Department of Physics and Electronics, Christ University, Karnataka, Bengaluru, 560029, India