Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Development of classical swine fever virus E2-protein based indirect ELISA for detection of antibodies against the virus in pigs Subject The topic of the resource Classical swine fever; CSFV; E2; ELISA Description An account of the resource Classical swine fever (CSF) is an economically important and highly contagious disease of pigs caused by CSF virus, genus Pestivirus. Serological diagnosis of the disease is highly valuable for surveillance and thereby containment of spread of the disease. In this study, we have demonstrated the development of CSFV envelope glycoprotein E2-based indirect ELISA (E2-iELISA) for the detection of CSFV specific antibodies. The full-length E2 protein was expressed in E. coli and the purified protein was used as a coating antigen in indirect ELISA for detecting CSFV specific antibodies in pigs. A panel of 506 pig sera samples was used to validate the ELISA and the results were highly comparable to the results obtained with the commercial antibody detection kit (PrioCHECK CSFV Ab kit). The in-house E2-iELISA demonstrated high diagnostic sensitivity (95.4%) and specificity (95.5%), highlighting its potential application for sero-surveillance or monitoring of the disease in the swine population. The Author(s), under exclusive licence to Springer Nature B.V. 2024. Creator An entity primarily responsible for making the resource Gopinath S.; Hosamani M.; Joseph B.V.; Patil S.S. Source A related resource from which the described resource is derived Veterinary Research Communications, Vol-48, No. 5, pp. 3121-3129. Publisher An entity responsible for making the resource available Springer Science and Business Media B.V. Date A point or period of time associated with an event in the lifecycle of the resource 2024-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1007/s11259-024-10482-1" target="_blank" rel="noreferrer noopener">https://doi.org/10.1007/s11259-024-10482-1</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85200128199&amp;doi=10.1007%2Fs11259-024-10482-1&amp;partnerID=40&amp;md5=073c256ceb37f4ea3308515c9bb1fbe3" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85200128199&amp;doi=10.1007%2fs11259-024-10482-1&amp;partnerID=40&amp;md5=073c256ceb37f4ea3308515c9bb1fbe3</a> Rights Information about rights held in and over the resource Restricted Access Relation A related resource ISSN: 1657380; PubMed ID: 39088127; CODEN: VRCOD Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Gopinath S., CHRIST UNIVERSITY, Karnataka, Bengaluru, 560029, India; Hosamani M., ICAR- Indian Veterinary Research Institute, Karnataka, Bengaluru, 560024, India; Joseph B.V., CHRIST UNIVERSITY, Karnataka, Bengaluru, 560029, India; Patil S.S., ICAR- National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Karnataka, Bengaluru, 560064, India