Appraisal of the potential of endophytic bacterium Bacillus amyloliquefaciens from Alternanthera philoxeroides: A triple approach to heavy metal bioremediation, diesel biodegradation, and biosurfactant production
Alternanthera philoxeroides; Bacillus amyloliquefaciens; Bioremediation; Biosurfactant, Diesel biodegradation; Endophyte; Heavy metal
Endophytic microbes have been associated with many positive traits due to their endurance mechanisms. The current study was designed at exploring the potential of the endophytic bacterium Bacillus amyloliquefaciens MEBAphL4 isolated from Alternanthera philoxeroides for biosurfactant production and bioremediation efficiency. This endophyte, isolated from the polluted Madiwala lake in Bangalore, displayed elevated resistance to Cr and Pb till 2000 mg/L. The metal removal efficiency was found to be higher for Cr (25.7 %) at pH 6 and for Pb (92.3 %) at pH 9. Further, the present study also describes biosurfactant production with good emulsification ability (E24-52 %) and stability over a range of pH (8?12), temperature (2040C) and salinity (515 %). Biosurfactant production was enhanced 1.18-fold using the Response Surface Methodology approach and characterised by Fourier Transformation Infra-red Spectroscopy and Ultra-Performance Liquid Chromatography- Mass Spectrometry showing the presence of lipopeptides, fengycin, iturin and surfactin of molecular weights 1463.65, 1043.44 and 1012.56 Da respectively. The potential application of the biosurfactant in degrading various hydrocarbons was evaluated, demonstrating its effectiveness in bioremediation of oil-contaminated sites. Specifically, diesel biodegradation was measured at 56.460.95 %. These findings underscore the potential of B. amyloliquefaciens in environmental applications such as heavy metal biosorption and the bioremediation of contaminated sites, particularly those affected by oil spills and correlates to UN SDG6 of clean water and sanitation. 2024 Elsevier Ltd
Biswas S.; Jayaram S.; Philip I.; Balasubramanian B.; Pappuswamy M.; BarcelD.; Chelliapan S.; Kamyab H.; Sarojini S.; Vasseghian Y.
Journal of Environmental Chemical Engineering, Vol-12, No. 5
https://doi.org/10.1016/j.jece.2024.113454 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85197601720&doi=10.1016%2fj.jece.2024.113454&partnerID=40&md5=849c73571cb3c692ecc84ebe98500e93
Biswas S., Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, India, Equal Contribution.; Jayaram S., Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, India; Philip I., Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, India; Balasubramanian B., Department of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul, 05006, South Korea, Equal Contribution.; Pappuswamy M., Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, India; BarcelD., Chemistry and Physics Department, University of Almeria, Almer, 04120, Spain; Chelliapan S., Department of Smart Engineering and Advanced Technology, Faculty of Artificial Intelligence, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, Kuala Lumpur, 54100, Malaysia; Kamyab H., Department of Biomaterials, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, 600 077, India, Faculty of Architecture and Urbanism, UTE University, Calle Rumipamba S/N and Bourgeois, Quito, Ecuador; Sarojini S., Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, India; Vasseghian Y., Department of Chemical Engineering and Material Science, Yuan Ze University, Taiwan