Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A dual-functional rhodamine B and azo-salicylaldehyde derivative for the simultaneous detection of copper and hypochlorite: synthesis, biological applications and theoretical insights Description An account of the resource A multifunctional rhodamine derivative containing azo-salicylaldehyde (BBS) was designed and synthesized as a colorimetric and fluorescence turn-on probe for the selective detection of copper cations (Cu2+) and hypochlorite anions (OCl?) in aqueous media. In the presence of Cu2+, the probe BBS exhibited turn-on absorption and fluorescence change at 554 nm and 585 nm, respectively. The binding mechanism of BBS with Cu2+ induces the opening of a spirolactam ring in the rhodamine moiety by the formation of a metal-ligand complex, achieving 10-fold enhancement in fluorescence and quantum yield, along with a binding constant of 1 104 M?1 and a detection limit of 2.61 ?M. Addition of OCl? enhanced the absorbance and fluorescence intensities at 520 nm and 575 nm, respectively. The probe BBS underwent hypochlorite-mediated oxidation, followed by hydrolysis, resulting in the formation of rhodamine B itself, which is detectable by the naked eye via the color and fluorescence enhancement by 11-fold with a high quantum yield and a detection limit of 1.96 ?M. For practical applications, sensor BBS can be used to detect Cu2+ in water samples and on cotton swabs. For biological applications, the interaction of the BBS-Cu(ii) complex with transport proteins such as bovine serum albumin (BSA) and ct-DNA was investigated through UV-vis and fluorescence titration experiments. Additionally, the structural optimization of BBS and the BBS-Cu(ii) complex was demonstrated using DFT, and the interactions of the BBS-Cu(ii) complex with BSA and ct-DNA were analysed through theoretical docking studies. Bioimaging studies were conducted by capturing fluorescence images of BBS with Cu2+ and OCl? in a physiological medium containing living plant tissue using green gram seeds. 2024 The Royal Society of Chemistry. Creator An entity primarily responsible for making the resource Vishnu S.; Das A.K.; Bylappa Y.; Nag A.; Dolai M. Source A related resource from which the described resource is derived Analytical Methods, Vol-16, No. 47, pp. 8164-8178. Publisher An entity responsible for making the resource available Royal Society of Chemistry Date A point or period of time associated with an event in the lifecycle of the resource 2024-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1039/d4ay01758g" target="_blank" rel="noreferrer noopener">https://doi.org/10.1039/d4ay01758g</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85208956278&amp;doi=10.1039%2Fd4ay01758g&amp;partnerID=40&amp;md5=4083f93d6704fbcc8c8827e2eb243723" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85208956278&amp;doi=10.1039%2fd4ay01758g&amp;partnerID=40&amp;md5=4083f93d6704fbcc8c8827e2eb243723</a> Rights Information about rights held in and over the resource Restricted Access Relation A related resource ISSN: 17599660; PubMed ID: 39508779 Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Vishnu S., Department of Chemistry, Christ University, Hosur Road, Karnataka, Bangalore, 560029, India; Das A.K., Department of Chemistry, Christ University, Hosur Road, Karnataka, Bangalore, 560029, India; Bylappa Y., Department of Life Science, Christ University, Hosur Road, Karnataka, Bangalore, 560029, India; Nag A., Department of Life Science, Christ University, Hosur Road, Karnataka, Bangalore, 560029, India; Dolai M., Department of Chemistry, Prabhat Kumar College, Purba Medinipur, WB, Contai, 721404, India