Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Pemetrexed loaded gold nanoparticles as cytotoxic and apoptosis inducers in lung cancer cells through ROS generation and mitochondrial dysfunction pathway Subject The topic of the resource apoptosis; gold nanoparticles; lung cancer; mitochondrial damage; Pemetrexed; ROS generation Description An account of the resource Supramolecular nanoparticles containing peptides and drugs have recently gained recognition as an effective tumor treatment drug delivery system. A multitarget drug termed pemetrexed is effective against various cancers, including nonsmall cell lung cancer. The work aims to establish the capability of pemetrexed gold nanoparticles (PEM-AuNPs) to induce apoptosis and explore molecular changes. X-ray diffraction, Fourier-transform infrared spectroscopy, ultravioletvisible spectroscopy, scanning electron microscope, and transmission electron microscope were used to investigate the synthesized nanoparticles. The MTT assay was utilized to investigate the anticancer properties of PEM-AuNPs at varying concentrations (50, 100, and 200M). PEM-AuNPs demonstrated a decrease in cell viability with 55.87%, 43.04%, and 25.59% for A549 cells and 54.31%, 37.40%, and 25.84% for H1299 cells at the respective concentrations. To assess apoptosis and perform morphological analysis, diverse biochemical staining techniques, including acridine orange-ethidium bromide and 4?,6-diamidino-2-phenylindole nuclear staining assays, were employed. Additionally, 2?,7?-dichlorofluorescein diacetate staining confirmed the induction of reactive oxygen species generation, while JC-1 staining validated the impact on the mitochondrial membrane at the IC50 concentration of PEM-AuNPs. Thus, the study demonstrated that the synthesized PEM-AuNPs exhibited enhanced anticancer activity against both A549 and H1299 cells. 2024 International Union of Biochemistry and Molecular Biology, Inc. Creator An entity primarily responsible for making the resource Baby Shakila P.; Periasamy T.; Alarfaj A.A.; Arulselvan P.; Ravindran R.; Suriyaprakash J.; Thangavelu I. Source A related resource from which the described resource is derived Biotechnology and Applied Biochemistry, Vol-71, No. 4, pp. 779-790. Publisher An entity responsible for making the resource available John Wiley and Sons Inc Date A point or period of time associated with an event in the lifecycle of the resource 2024-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1002/bab.2576" target="_blank" rel="noreferrer noopener">https://doi.org/10.1002/bab.2576</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85187516810&amp;doi=10.1002%2Fbab.2576&amp;partnerID=40&amp;md5=4ab787b1c57b49a449e5fd39401baee6" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85187516810&amp;doi=10.1002%2fbab.2576&amp;partnerID=40&amp;md5=4ab787b1c57b49a449e5fd39401baee6</a> Rights Information about rights held in and over the resource Restricted Access Relation A related resource ISSN: 8854513; PubMed ID: 38475937; CODEN: BABIE Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Baby Shakila P., Department of Biochemistry, Vivekanandha College of Arts and Sciences for Women (Autonomous), Tiruchengode, Tamilnadu, Namakkal, India; Periasamy T., Department of Biochemistry, Muthayammal College of Arts and Science (Autonomous), Namakkal, India; Alarfaj A.A., Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia; Arulselvan P., Department of Chemistry, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India; Ravindran R., Preclinical Department, Faculty of Medicine, Universiti Kuala Lumpur, Royal College of Medicine Perak, Ipoh, Malaysia; Suriyaprakash J., Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou, China; Thangavelu I., Department of Chemistry, CHRIST (Deemed to be University), Bangalore, India