Sustainable biodegradation of textile dye reactive blue 222 by the novel strain Enterobacter CU2004, isolated from the industrial waste: A design of experiment based optimization study and characterisation of metabolites
- Title
- Sustainable biodegradation of textile dye reactive blue 222 by the novel strain Enterobacter CU2004, isolated from the industrial waste: A design of experiment based optimization study and characterisation of metabolites
- Creator
- Lakshmaiah V.V.; Nag A.; Gotekar S.; More S.; Jayanna S.K.
- Description
- Reactive Blue 222 (RB222) is widely used in textile industries and hence a common recalcitrant pollutant in the industrial effluent. Bioremediation of this dye is of significance as its one of the complex dyes with high molecular weight. In the present study, we isolated a novel bacterial strain Enterobacter CU2004 from the industrial waste and characterize using16S rRNA gene sequencing. Its potential to dye degradation was evaluated in a simple minimal salt media with the parameters namely dye concentration (1001000 ppm), pH (49), temperature (1555C), Carbon source (Lactose, Sucrose, Glucose, Starch, and Fructose), and Nitrogen source (Casein, Yeast extract, Peptone, Tryptone, Ammonium sulphate, and Urea) in a 24 h culture. Finally, data obtained were extended to design of experiment based optimization for the degradation efficacy of Enterobacter CU2004 and to validated design space was established. The novelty is in optimizing the design space parameters for highest percentage of degradation ?90% by the bacterial isolate Enterobacter CU2004 were finalized as 3037C temperature, 133249 ppm dye concentration, Lactose as Carbon source, Yeast extract as Nitrogen source, and the pH as 8. Microbial dye degradation was confirmed by FTIR, HPLC and GCMS studies. Further studies revealed the dye intermediates and the potential of Enterobacter CU2004 toward the degradation of complex, high molecular weight industrial dye RB222. 2024 Vasantha Veerappa Lakshmaiah, et al.
- Source
- Journal of Applied Biology and Biotechnology, Vol-12, No. 3, pp. 257-267.
- Date
- 2024-01-01
- Publisher
- Open Science Publishers LLP Inc.
- Subject
- Biodegradation; Bioremediation; Design of experiment; Enterobacter; Reactive blue 222; Statistical modeling
- Coverage
- Lakshmaiah V.V., Department of Microbiology, School of Basic and Applied Sciences, Dayananda Sagar University, Kumaraswamy Layout, Bengaluru, 560078, India; Nag A., Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, 560029, India; Gotekar S., Department of Chemistry, Smt Devkiba Mohanshansinhji Chauhan College of Commerce and Science (University of Mumbai), Dadra and Nagar Haveli, India; More S., Department of Biochemistry, School of Basic and Applied Sciences, Dayananda Sagar University, Kumaraswamy Layout, Bengaluru, 560078, India; Jayanna S.K., Department of Microbiology, School of Basic and Applied Sciences, Dayananda Sagar University, Kumaraswamy Layout, Bengaluru, 560078, India
- Rights
- All Open Access; Gold Open Access
- Relation
- ISSN: 2347212X
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Lakshmaiah V.V.; Nag A.; Gotekar S.; More S.; Jayanna S.K., “Sustainable biodegradation of textile dye reactive blue 222 by the novel strain Enterobacter CU2004, isolated from the industrial waste: A design of experiment based optimization study and characterisation of metabolites,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 25, 2025, https://archives.christuniversity.in/items/show/13136.