Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Functional characterization of Malabar grouper (Epinephelus malabaricus) interferon regulatory factor 9 involved in antiviral response Subject The topic of the resource ADAR families; Epinephelus malabaricus; Interferon; IRF9; Malabar grouper; Nervous necrosis virus Description An account of the resource IRF9 is a crucial component in the JAK-STAT pathway. IRF9 interacts with STAT1 and STAT2 to form IFN-I-stimulated gene factor 3 (ISGF3) in response to type I IFN stimulation, which promotes ISG transcription. However, the mechanism by which IFN signaling regulates Malabar grouper (Epinephelus malabaricus) IRF9 is still elusive. Here, we explored the nd tissue-specific mRNA distribution of the MgIRF9 gene, as well as its antiviral function in E. malabaricus. MgIRF9 encodes a protein of 438 amino acids with an open reading frame of 1317 base pairs. MgIRF9 mRNA was detected in all tissues of a healthy M. grouper, with the highest concentrations in the muscle, gills, and brain. It was significantly up-regulated by nervous necrosis virus infection and poly (I:C) stimulation. The gel mobility shift test demonstrated a high-affinity association between MgIRF9 and the promoter of zfIFN in vitro. In GK cells, grouper recombinant IFN-treated samples showed a significant response in ISGs and exhibited antiviral function. Subsequently, overexpression of MgIRF9 resulted in a considerable increase in IFN and ISGs mRNA expression (ADAR1, ADAR1-Like, and ADAR2). Co-immunoprecipitation studies demonstrated that MgIRF9 and STAT2 can interact in vivo. According to the findings, M. grouper IRF9 may play a role in how IFN signaling induces ISG gene expression in grouper species. 2024 Elsevier B.V. Creator An entity primarily responsible for making the resource Periyasamy T.; Ming-Wei L.; Velusamy S.; Ahamed A.; Khan J.M.; Pappuswamy M.; Viswakethu V. Source A related resource from which the described resource is derived International Journal of Biological Macromolecules, Vol-266 Publisher An entity responsible for making the resource available Elsevier B.V. Date A point or period of time associated with an event in the lifecycle of the resource 2024-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1016/j.ijbiomac.2024.131282" target="_blank" rel="noreferrer noopener">https://doi.org/10.1016/j.ijbiomac.2024.131282</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85189558428&amp;doi=10.1016%2Fj.ijbiomac.2024.131282&amp;partnerID=40&amp;md5=61bd5d15eaaa21f9870689c76542aa3e" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85189558428&amp;doi=10.1016%2fj.ijbiomac.2024.131282&amp;partnerID=40&amp;md5=61bd5d15eaaa21f9870689c76542aa3e</a> Rights Information about rights held in and over the resource Restricted Access Relation A related resource ISSN: 1418130; PubMed ID: 38565369; CODEN: IJBMD; LS; 2023-2024; Vol-1; 0934-0942 Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Periyasamy T., Laboratory of Molecular Virology and Immunology, Department of Aquaculture, The College of Life Science, National Taiwan Ocean University, Keelung, 202, Taiwan, Department of Biotechnology, Nehru Arts and Science College, Tamil Nadu, Coimbatore, 641105, India; Ming-Wei L., Laboratory of Molecular Virology and Immunology, Department of Aquaculture, The College of Life Science, National Taiwan Ocean University, Keelung, 202, Taiwan, Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, 202, Taiwan; Velusamy S., Department of Biotechnology, Nehru Arts and Science College, Tamil Nadu, Coimbatore, 641105, India; Ahamed A., Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia; Khan J.M., Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, 11451, Saudi Arabia; Pappuswamy M., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bangalore, 560029, India; Viswakethu V., Department of Biotechnology, Nehru Arts and Science College, Tamil Nadu, Coimbatore, 641105, India