Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Isolation and characterization of polyhydroxyalkanoate producing halotolerant Bacillus subtilis SG1 using marine water samples collected from Calicut coast, Kerala Subject The topic of the resource Bacillus subtilis; Bacterial biopolymer; Halotolerance; Polyhydroxyalkanoates Description An account of the resource Halotolerant bacterial strain isolated from the Calicut coast, Kerala, India, was screened for its potential ability to synthesize polyhydroxyalkanoates (PHA) using Sudan black B and Nile blue staining. The quantitative analysis for PHA production was done in modified M9 media and a PHA yield of about 1.52 g/L was observed with the most potential isolate SG1. Further, the biochemical and molecular characterization of the PHA-producing halotolerant bacteria was done using standard biochemical tests and 16 s ribosomal RNA sequencing respectively and the isolate was identified to be Bacillus subtilis SG1. Further, the PHA recovery was done using solvent extraction method employing acetone and diethyl ether followed by precipitation using chloroform with a maximum PHA yield of 1.52 g/L. Further, the material properties of the extracted polymer were studied using Fourier transform infrared, X-ray diffraction, nuclear magnetic resonance, thermo gravimetric analysis, and differential scanning calorimetry analysis. Further investigations are necessary to optimize PHA production and to carry out its application study in various fields. 2024 Sneha Grigary, et al. Creator An entity primarily responsible for making the resource Grigary S.; Umesh M.; Mani V.M. Source A related resource from which the described resource is derived Journal of Applied Biology and Biotechnology, Vol-12, No. 2, pp. 282-288. Publisher An entity responsible for making the resource available Open Science Publishers LLP Inc. Date A point or period of time associated with an event in the lifecycle of the resource 2024-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.7324/JABB.2024.143107" target="_blank" rel="noreferrer noopener">https://doi.org/10.7324/JABB.2024.143107</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85185928829&amp;doi=10.7324%2FJABB.2024.143107&amp;partnerID=40&amp;md5=8e0a260f2c09c4cc759e1d4e143cd2e0" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85185928829&amp;doi=10.7324%2fJABB.2024.143107&amp;partnerID=40&amp;md5=8e0a260f2c09c4cc759e1d4e143cd2e0</a> Rights Information about rights held in and over the resource All Open Access; Gold Open Access Relation A related resource ISSN: 2347212X | LS; 2023-2024; Vol-2; 0225-0231 Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Grigary S., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Umesh M., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Mani V.M., Department of Biotechnology, RVS College of Arts and Science, Tamil Nadu, Coimbatore, India