Development and Validation of the UV-Spectrophotometric Method for the Determination of Cordycepin, a Nucleoside
- Title
- Development and Validation of the UV-Spectrophotometric Method for the Determination of Cordycepin, a Nucleoside
- Creator
- Krishna K.V.; Devasia J.; Malaviya A.
- Description
- The primary, bioactive component of Cordyceps militaris, known as cordycepin (3'-deoxyadenosine), has been utilized extensively as a traditional medicinal ingredient and a nutritious diet in Asian nations. To determine the amount of cordycepin in the C. militaris, high-performance liquid chromatography (HPLC) is shown to be an effective, sensitive, and straightforward analytical technique. Other methods for estimating cordycepin include near infrared spectroscopy and capillary electrophoresismass spectrometry. The UV-Vis spectroscopy approach was used to quantify cordycepin, and the results were statistically confirmed and compared with an HPLC method. For linearity, repeatability, precision, reproducibility, limit of detection and limit of quantification, the method was verified. The established approach can be utilized for routine, quality control analysis of cordycepin, as it is easy to use, sensitive, accurate, precise, repeatable, and most importantly, cost-effective. Springer Science+Business Media, LLC, part of Springer Nature 2024.
- Source
- Journal of Applied Spectroscopy, Vol-91, No. 1, pp. 228-235.
- Date
- 2024-01-01
- Publisher
- Springer
- Subject
- Cordycepin; Cordyceps militaris; high-performance liquid chromatography; UV-Vis spectroscopy
- Coverage
- Krishna K.V., Applied and Industrial Biotechnology Laboratory, Christ (deemed-to-be University), Hosur Road, Karnataka, Bangalore, India; Devasia J., Adjunct Faculty, Division of Life Sciences, Gyeongsang National University, Gyeongsang, South Korea; Malaviya A., QuaLife Biotech Pvt Ltd, Bangalore, India
- Rights
- Restricted Access
- Relation
- ISSN: 219037
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Krishna K.V.; Devasia J.; Malaviya A., “Development and Validation of the UV-Spectrophotometric Method for the Determination of Cordycepin, a Nucleoside,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 25, 2025, https://archives.christuniversity.in/items/show/13253.