Fluorescence bioimaging applications of europium-doped strontium aluminate nanoparticles
- Title
- Fluorescence bioimaging applications of europium-doped strontium aluminate nanoparticles
- Creator
- Thomas N.M.; George N.; Viji M.O.; Bins K.C.; Chakrapani P.S.B.; Anila E.I.
- Description
- Fluorescence bioimaging is widely used for physiological studies to visualise intercellular molecular events due to its highly selective, sensitive, and non-destructive nature. However, its application in in vivo live imaging is often limited by the scarcity of biocompatible fluorescent probes possessing optimal properties. Our study focuses on developing europium-based nanoparticles for in vivo bioimaging, especially imaging of plants. Eu-doped strontium aluminate nanoparticles were synthesised through a conventional solid-state reaction. Structural characterisation of samples using XRD confirmed the prevalence of SrAl2O4 as the prominent phase. The FTIR spectrum, SEM and TEM images were recorded for further characterization. Photoluminescence studies showed orange red emission of sample. The antibacterial activity of the nanophosphors was studied, demonstrating no antibacterial activity against Escherichia coli and Pseudomonas aeruginosa. Furthermore, in vitro cytotoxicity studies conducted using Neuro-2A cells showed no indications of cytotoxicity associated with europium doped strontium aluminate nanoparticles. When incorporated into the plant tissue culture medium, these nanoparticles were found to have no effect on seed germination and plant growth, and it demonstrated no phytotoxicity. Imaging studies have shown the uptake of nanoparticles by plants and their subsequent transport through the vascular system. Our results emphasise the direct integration of nanophosphors into plant tissues from the growth medium, eliminating the necessity for traditional staining methods in fluorescence bioimaging. Incorporation of nanophosphors into living organisms holds promise for non-invasive and long-term fluorescence imaging, with potential applications in biological studies and diagnostics. The outstanding fluorescence properties and biocompatibility of europium doped strontium aluminate nanoparticles broaden its potential for various applications in fluorescence bioimaging. 2024 Elsevier Ltd and Techna Group S.r.l.
- Source
- Ceramics International
- Date
- 2024-01-01
- Publisher
- Elsevier Ltd
- Subject
- Biocompatibility; Europium; Fluorescence bioimaging; Nanoparticles; Strontium aluminate
- Coverage
- Thomas N.M., Department of Physics, Morning Star Home Science College, Angamaly, Kerala, Ernakulam, 683573, India, Center for Nano-Bio Polymer Science and Technology, Department of Physics, St. Thomas College, Palai, Arunapuram, Kerala, Kottayam, 686574, India, Optoelectronic and Nanomaterials' Research Lab, Department of Physics, Union Christian College, Aluva, Kerala, Ernakulam, 683102, India; George N., Department of Biotechnology, St. Joseph's College (Autonomous), Irinjalakuda (Affiliated to the University of Calicut), Kerala, 680121, India; Viji M.O., Department of Biotechnology, St. Joseph's College (Autonomous), Irinjalakuda (Affiliated to the University of Calicut), Kerala, 680121, India; Bins K.C., Centre for Neuroscience, Department of Biotechnology, Cochin University for Science and Technology, Kerala, Kochi, 682022, India; Chakrapani P.S.B., Centre for Neuroscience, Department of Biotechnology, Cochin University for Science and Technology, Kerala, Kochi, 682022, India; Anila E.I., Optoelectronic and Nanomaterials' Research Lab, Department of Physics, Union Christian College, Aluva, Kerala, Ernakulam, 683102, India, Department of Physics and Electronics, Christ (Deemed to be University), Karnataka, Bangalore, 560029, India
- Rights
- Restricted Access
- Relation
- ISSN: 2728842; CODEN: CINND
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Thomas N.M.; George N.; Viji M.O.; Bins K.C.; Chakrapani P.S.B.; Anila E.I., “Fluorescence bioimaging applications of europium-doped strontium aluminate nanoparticles,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 24, 2025, https://archives.christuniversity.in/items/show/13385.