Green Synthesis of Hydroxyapatite Nanoparticles Using Semecarpus anacardium Linn leaf Extract: Examination of Anticancer Activity and its Apoptosis Induction
- Title
- Green Synthesis of Hydroxyapatite Nanoparticles Using Semecarpus anacardium Linn leaf Extract: Examination of Anticancer Activity and its Apoptosis Induction
- Creator
- Rekha M.M.; Ojha V.; Mewada B.; Gupta S.; Chinnathambi A.; Alahmadi T.A.; Arulselvan P.; Thangavelu I.
- Description
- Nanotechnology and biomedical sciences enable diverse molecular and cellular applications. Plant-mediated biosynthesis of nanoparticles, a green chemistry approach, offers a cost-effective, eco-friendly alternative to traditional methods. This study focuses on developing hydroxyapatite nanoparticles (HA-NPs) using Semecarpus anacardium Linn (SAL) leaf extract (termed SAL@HA-NPs) as a capping agent and reducing agent. The presence of needle-shaped nanostructures was verified using SEM and TEM investigation. The presence of well-defined rings in the selected area electron diffraction (SAED) patterns provided evidence for the polycrystalline nature of the SAL@HA-NPs). The XRD spectrum exhibited clear peaks that closely corresponded to the hexagonal patterns of HA, indicating a mean crystalline diameter of 54.25nm. The FTIR analysis revealed the presence of biomolecules from Semecarpus anacardium Linn leaves on the surface of the nanoparticles. The suspension of SAL@HA-NPs displayed a polydispersity index of 0.445 and demonstrated excellent stability, as indicated by the zeta potential of -32.2 mV, as observed in the DLS tests. The SAL@HA-NPs exhibited a harmful effect on the HeLa cervical and HepG2 liver cancer cells, with an IC50 value of 52g/mL. Fluorescence microscopy revealed the deformation of the damaged cell membrane, fragmentation, and cell death following treatment with SAL@HA-NPs. The Annexin V-FITC and PI staining confirmed the mode of apoptosis by flow cytometric analysis. Thus, the SAL@HA-NPs acquired in this study could have a crucial impact on the biomedical domain of cancer treatment. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.
- Source
- Journal of Inorganic and Organometallic Polymers and Materials
- Date
- 2024-01-01
- Publisher
- Springer
- Subject
- Anticancer activity; Apoptosis; Green fabrication; Hydroxyapatite
- Coverage
- Rekha M.M., Department of Chemistry and Biochemistry, JAIN (Deemed-to-be University), Karnataka, Bangalore, 560069, India; Ojha V., Centre for Research and Development, Chitkara University, Himachal Pradesh, 174103, India; Mewada B., Department of Mechanical Engineering, PIT, Parul University, Gujarat, Vadodara, 31760, India; Gupta S., Department of Sciences, Vivekananda Global University, Jaipur, 303012, India; Chinnathambi A., Department of Botany and Microbiology, College of Science, King Saud University, PO Box - 2455, Riyadh, 11451, Saudi Arabia; Alahmadi T.A., Department of Pediatrics, College of Medicine and King Khalid University Hospital, King Saud University, PO Box-2925, Medical City, Riyadh, 11461, Saudi Arabia; Arulselvan P., Department of Chemistry, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 602105, India; Thangavelu I., Department of Chemistry, CHRIST (Deemed to be University), Karnataka, Bangalore, 560029, India
- Rights
- Restricted Access
- Relation
- ISSN: 15741443
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Rekha M.M.; Ojha V.; Mewada B.; Gupta S.; Chinnathambi A.; Alahmadi T.A.; Arulselvan P.; Thangavelu I., “Green Synthesis of Hydroxyapatite Nanoparticles Using Semecarpus anacardium Linn leaf Extract: Examination of Anticancer Activity and its Apoptosis Induction,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 28, 2025, https://archives.christuniversity.in/items/show/13414.