Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Citrus Medica-derived Fluorescent Carbon Dots for the Imaging of Vigna Radiate Root Cells Subject The topic of the resource Bioimaging; Carbon dots; Citrus medica; Fluorescence; Hydrothermal Method; Root Cells Description An account of the resource Bio-imaging is a crucial tool for researchers in the fields of cell biology and developmental biomedical sector. Among the various available imaging techniques, fluorescence based imaging stands out due to its high sensitivity and specificity. However, traditional fluorescent materials used in biological imaging often suffer from issues such as photostability and biocompatibility. Moreover, plant tissues contain compounds that cause autofluorescence and light scattering, which can hinder fluorescence microscopy effectiveness. This study explores the development of fluorescent carbon dots (Cm-CDs) synthesized from Citrus medica fruit extract for the fluorescence imaging of Vigna radiata root cells. The successful synthesis of CDs with an average size of 6.7nm is confirmed by Transmission Electron Microscopy (TEM). The X-ray diffraction (XRD) analysis and raman spectroscopy indicated that the obtained CDs are amorphous in nature. The presence of various functional groups on the surface of CDs were identified by Fourier transform infrared (FTIR) spectra. The optical characteristics of Cm-CDs were studied by UV-Visible spectroscopy and photoluminescence spectroscopy. Cm-CDs demonstrated strong excitation-dependent fluorescence, good solubility, and effective penetration in to the Vigna radiata root cells with multicolor luminescence, and addressed autofluorescence issues. Additionally, a comparative analysis determined the optimal concentration for high-resolution, multi-color root cell imaging, with Cm-CD2 (2.5mg/ml) exhibiting the highest photoluminescence (PL) intensity. These findings highlight the potential of Cm-CDs in enhancing direct endocytosis and overcoming autofluorescence in plant cell imaging, offering promising advancements for cell biology research. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024. Creator An entity primarily responsible for making the resource Varghese M.; Bylappa Y.; Nag A.; Kumbhakar P.; Balachandran M. Source A related resource from which the described resource is derived Journal of Fluorescence Publisher An entity responsible for making the resource available Springer Date A point or period of time associated with an event in the lifecycle of the resource 2024-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1007/s10895-024-03790-x" target="_blank" rel="noreferrer noopener">https://doi.org/10.1007/s10895-024-03790-x</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85195444999&amp;doi=10.1007%2Fs10895-024-03790-x&amp;partnerID=40&amp;md5=1b870777dfb19f014df3e53723da0ea2" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85195444999&amp;doi=10.1007%2fs10895-024-03790-x&amp;partnerID=40&amp;md5=1b870777dfb19f014df3e53723da0ea2</a> Rights Information about rights held in and over the resource Restricted Access Relation A related resource ISSN: 10530509; CODEN: JOFLE Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Varghese M., Department of Physics and Electronics, Christ University, Karnataka, Bengaluru, 560029, India; Bylappa Y., Department of Life Sciences, Christ University, Karnataka, Bengaluru, 560029, India; Nag A., Department of Life Sciences, Christ University, Karnataka, Bengaluru, 560029, India; Kumbhakar P., Department of Physics and Electronics, Christ University, Karnataka, Bengaluru, 560029, India; Balachandran M., Department of Physics and Electronics, Christ University, Karnataka, Bengaluru, 560029, India