Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Evidence of microRNAs origination from chloroplast genome and their role in regulating Photosystem II protein N (psbN) mRNA Subject The topic of the resource chloroplast; miRNA; nuclei; photosystem II protein N; RACE-PCR Description An account of the resource The microRNAs are endogenous, regulating gene expression either at the DNA or RNA level. Despite the availability of extensive studies on microRNA generation in plants, reports on their abundance, biogenesis, and consequent gene regulation in plant organelles remain naVve. Building on previous studies involving pre-miRNA sequencing in Abelmoschus esculentus, we demonstrated that three putative microRNAs were raised from the chloroplast genome. In the current study, we have characterized the genesis of these three microRNAs through a combination of bioinformatics and experimental approaches. The gene sequence for a miRNA, designated as AecpmiRNA1 (A. esculentus chloroplast miRNA), is potentially located in both the genomic DNA, i.e., nuclear and chloroplast genome. In contrast, the gene sequences for the other two miRNAs (AecpmiRNA2 and AecpmiRNA3) are exclusively present in the chloroplast genome. Target prediction revealed many potential mRNAs as targets for AecpmiRNAs. Further analysis using 5N RACE-PCR determined the AecpmiRNA3 binding and cleavage site at the photosystem II protein N (psbN). These results indicate that AecpmiRNAs are generated from the chloroplast genome, possessing the potential to regulate mRNAs arising from chloroplast gene(s). On the other side, the possibility of nuclear genome-derived mRNA regulation by AecpmiRNAs cannot be ruled out. 2024, Termedia Publishing House Ltd.. All rights reserved. Creator An entity primarily responsible for making the resource Anand A.; Chauhan S.; Chodon A.; Vimala Kumar K.V.; Saravanakumar S.; Pandi G. Source A related resource from which the described resource is derived Biotechnologia, Vol-105, No. 1, pp. 19-32. Publisher An entity responsible for making the resource available Termedia Publishing House Ltd. Date A point or period of time associated with an event in the lifecycle of the resource 2024-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.5114/bta.2024.135639" target="_blank" rel="noreferrer noopener">https://doi.org/10.5114/bta.2024.135639</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85190791270&amp;doi=10.5114%2Fbta.2024.135639&amp;partnerID=40&amp;md5=cf08c7db4dbc1bdf231f3190247311bb" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85190791270&amp;doi=10.5114%2fbta.2024.135639&amp;partnerID=40&amp;md5=cf08c7db4dbc1bdf231f3190247311bb</a> Rights Information about rights held in and over the resource All Open Access; Gold Open Access; Green Open Access Relation A related resource ISSN: 8607796|LS; 2023-2024; Vol-1; 0236-0249 Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Anand A., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India, Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Chauhan S., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India; Chodon A., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India; Vimala Kumar K.V., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India; Saravanakumar S., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India; Pandi G., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India