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    <name>Article</name>
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          <name>Title</name>
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              <text>UV-C and gamma radiation mediated L-DOPA production from in-vitro cultures of Mucuna pruriens (L.) DC</text>
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          <name>Subject</name>
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              <text>Cell cultures; Gamma radiation; In-vitro elicitation; L-DOPA; Mucuna pruriens; UV-C radiation</text>
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          <name>Description</name>
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              <text>This is the first report on UV-C and gamma rays mediated in-vitro elicitation of L-DOPA from Mucuna pruriens (L.) DC. cell suspension cultures. Gamma and ultraviolet rays are used on plants to induce mutations which results in activation of defence cascades and production of secondary metabolites due to this abiotic stress. The in-vitro callus developed from 0.5mg/L picloram was suspended into liquid medium and exposed to different time intervals (0, 15, 30, 45 and 60min) of UV-C radiations. On the other hand, the seeds were directly exposed to different doses (25, 50, 100, 150 and 200Gy) of gamma radiations and these irradiated seeds were grown in-vitro from which callus and cell cultures were established. From all these in-vitro cultures, the anti-Parkinsons drug L-DOPA was quantified using HPLC. 60 and 30-minute exposure of UV-C radiations resulted in highest biomass (193.27g/L FW) and L-DOPA production (5.13mg/g DW) respectively both showing a 1.5-fold increase than the control. In gamma radiation studies, 100Gy (Gy) dose showed the highest (83%) seed germination rate, 150Gy increased the in-vitro root and shoot length, while 100Gy increased the biomass of the cell cultures. Also, 150Gy dose showed a 6.1, 2.6 and 2.4-fold increase in L-DOPA production in the in-vitro roots, in-vitro shoots, and cell suspension culture respectively when compared to the control. UV light exposure of 30min and 150Gy doses of gamma radiation showed a significant increase in L-DOPA production.  The Author(s) under exclusive licence to Society for Plant Research 2024.</text>
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              <text>Bhaskar R.; Nagella P.; Madhu A.; Suriyamurthy N.; Srinatha N.</text>
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              <text>Vegetos</text>
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              <text>Springer</text>
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          <name>Date</name>
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              <text>2024-01-01</text>
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              <text>&lt;a href="https://doi.org/10.1007/s42535-024-00842-2" target="_blank" rel="noreferrer noopener"&gt;https://doi.org/10.1007/s42535-024-00842-2&lt;/a&gt;
&lt;br /&gt;&lt;br /&gt;&lt;a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85186174081&amp;amp;doi=10.1007%2Fs42535-024-00842-2&amp;amp;partnerID=40&amp;amp;md5=6aa04ec0fa52eecebf33a240df38a9f2" target="_blank" rel="noreferrer noopener"&gt;https://www.scopus.com/inward/record.uri?eid=2-s2.0-85186174081&amp;amp;doi=10.1007%2fs42535-024-00842-2&amp;amp;partnerID=40&amp;amp;md5=6aa04ec0fa52eecebf33a240df38a9f2&lt;/a&gt;</text>
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              <text>Restricted Access</text>
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              <text>ISSN: 9704078</text>
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              <text>Online</text>
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              <text>English</text>
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              <text>Bhaskar R., Department of Life Sciences, CHRIST (Deemed to Be University), Hosur Road, Bangalore, 560 029, India; Nagella P., Department of Life Sciences, CHRIST (Deemed to Be University), Hosur Road, Bangalore, 560 029, India; Madhu A., Department of Physics, Dayananda Sagar College of Engineering, Kumarswamy layout, Bangalore, 560111, India; Suriyamurthy N., Health Physics Unit, Prototype Fast Breeder Reactor, BHAVINI, Kokilamedu, Kalpakkam, 603 102, India; Srinatha N., Department of Physics, RV Institute of Technology and Management, Bengaluru, 560 076, India</text>
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