Polyamines influence the production of Boeravinone-B from cell suspension cultures of Boerhavia diffusa L.

Title

Polyamines influence the production of Boeravinone-B from cell suspension cultures of Boerhavia diffusa L.

Creator

Sudheer W.N.; Nagella P.

Description

Boerhavia diffusa L. (Punarnava) is an ayurvedic herb with a wide range of phytochemicals and pharmacological activities that have been utilised since antiquity. Boeravinone-B (an isoflavone rotenoid molecule) is one of the most significant secondary metabolites produced in its plant body. Modern plant biotechnological tools have helped in the overproduction of desired plant secondary metabolites from in vitro cell and organ cultures. Elicitation is one such strategy employed for the production of phytochemicals. In the present study, polyamines like putrescine, spermine and spermidine have been used as elicitors for the production of Boeravinone-B. The cell suspension cultures of punarnava have been treated with polyamines at various concentrations, ranging from 0.5 to 20M, at various day intervals of 2, 4 and 6 before the harvesting. The fresh weights, dry weights of cell suspension cultures, their Boeravinone-B content and yield are evaluated. Among all the various polyamine treatments, 2.5M spermidine (SPD) on the 6th day before harvesting shows the highest Boeravinone-B content of 10.88 0.13mgg?1 DW and yield of 110.34mg L?1 respectively, which is a five-fold increment compared to the control cultures (2.16 0.06mgg?1 DW and 16.35mg L?1) respectively. The highest total phenolic content in the cell suspension cultures is observed with 1?M SPD on the 2nd day prior to harvesting (194.25 0.37mgg?1 DW), while the highest levels of flavonoids are observed with 2.5?M SPD on the 6th day before harvesting (86.85 0.26mgg?1 DW). 2023, The Author(s), under exclusive licence to Springer Nature B.V.

Source

Plant Cell, Tissue and Organ Culture, Vol-156, No. 1

Date

2024-01-01

Publisher

Springer Science and Business Media B.V.

Subject

Boeravinone-B; Boerhavia diffusa L; Cell suspension culture; Elicitation; Polyamines

Coverage

Sudheer W.N., Department of Life Sciences, CHRIST (Deemed to Be University), Bengaluru, 560029, India; Nagella P., Department of Life Sciences, CHRIST (Deemed to Be University), Bengaluru, 560029, India

Rights

Restricted Access

Relation

ISSN: 1676857; CODEN: PTCED

Format

Online

Language

English

Type

Article

Identifier

https://doi.org/10.1007/s11240-023-02629-w

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85179318031&doi=10.1007%2fs11240-023-02629-w&partnerID=40&md5=9375bd850f79325ab850bee39e852a3c