In silico analysis of NHP2 membrane protein, a novel vaccine candidate present in the RD7 region of Mycobacterium tuberculosis
- Title
- In silico analysis of NHP2 membrane protein, a novel vaccine candidate present in the RD7 region of Mycobacterium tuberculosis
- Creator
- Kootery K.P.; Sarojini S.
- Description
- Mycobacterium tuberculosis, the etiological agent of tuberculosis, is one of the trickiest pathogens. We have only a few protective shields, like the BCG vaccine against the pathogen, which itself has poor efficacy in preventing adult tuberculosis. Even though different vaccine trials for an alternative vaccine have been conducted, those studies have not shown much promising results. In the current study, advanced computational technology was used to study the potential of a novel hypothetical mycobacterial protein, identified by subtractive hybridization, to be a vaccine candidate. NHP2 (Novel Hypothetical Protein 2), housed in the RD7 region of the clinical strains of M. tuberculosis, was studied for its physical, chemical, immunological and structural properties using different computational tools. PFAM studies and Gene ontology studies depicted NHP2 protein to be functionally active with a possible antibiotic binding domain too. Different computational tools used to assess the toxicity, allergenicity and antigenicity of the protein indicated its antigenic nature. Immune Epitope Database (IEDB) tools were used to study the T and B cell determinants of the protein. The 3D structure of the protein was designed, refined and authenticated using bioinformatics tools. The validated tertiary structure of theprotein was docked against the TLR3 immune receptor to study the binding affinity and docking scores. Molecular dynamic simulation of the protein-protein complex formed were studied. NHP2 was found to activate host immune response against tubercle bacillus and could be explored as a potential vaccine in the fight against tuberculosis. 2023, The Author(s), under exclusive licence to Plant Science and Biodiversity Centre, Slovak Academy of Sciences (SAS), Institute of Zoology, Slovak Academy of Sciences (SAS), Institute of Molecular Biology, Slovak Academy of Sciences (SAS).
- Source
- Biologia, Vol-79, No. 1, pp. 355-372.
- Date
- 2024-01-01
- Publisher
- Springer Science and Business Media Deutschland GmbH
- Subject
- Gene ontology; Molecular docking; Molecular dynamics simulation; Mycobacterium tuberculosis; NHP2; PFAM; TLR3; Vaccine
- Coverage
- Kootery K.P., Department of Life Sciences, CHRIST (Deemed to Be University), 29, Bangalore, India; Sarojini S., Department of Life Sciences, CHRIST (Deemed to Be University), 29, Bangalore, India
- Rights
- Restricted Access
- Relation
- ISSN: 63088
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Kootery K.P.; Sarojini S., “In silico analysis of NHP2 membrane protein, a novel vaccine candidate present in the RD7 region of Mycobacterium tuberculosis,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 24, 2025, https://archives.christuniversity.in/items/show/13823.