Viscosity-sensitive and AIE-active bimodal fluorescent probe for the selective detection of OCl? and Cu2+: a dual sensing approach via DFT and biological studies using green gram seeds
- Title
- Viscosity-sensitive and AIE-active bimodal fluorescent probe for the selective detection of OCl? and Cu2+: a dual sensing approach via DFT and biological studies using green gram seeds
- Creator
- Kumar M.S.; Vishnu S.; Dolai M.; Nag A.; Bylappa Y.; Das A.K.
- Description
- A novel dual-mode viscosity-sensitive and AIE-active fluorescent chemosensor based on the naphthalene coupled pyrene (NCP) moiety was designed and synthesized for the selective detection of OCl? and Cu2+. In non-viscous media, NCP exhibited weak fluorescence; however, with an increase in viscosity using various proportions of glycerol, the fluorescence intensity was enhanced to 461 nm with a 6-fold increase in fluorescence quantum yields, which could be utilized for the quantitative determination of viscosity. Interestingly, NCP exhibited novel AIE characteristics in terms of size and growth in H2O-CH3CN mixtures with high water contents and different volume percentage of water, which was investigated using fluorescence, DLS study and SEM analysis. Interestingly, this probe can also be effectively employed as a dual-mode fluorescent probe for light up fluorescent detection of OCl? and Cu2+ at different emission wavelengths of 439 nm and 457 nm via chemodosimetric and chelation pathways, respectively. The fast-sensing ability of NCP towards OCl? was shown by a low detection limit of 0.546 ?M and the binding affinity of NCP with Cu2+ was proved by a low detection limit of 3.97 ?M and a high binding constant of 1.66 103 M?1. The sensing mechanism of NCP towards OCl? and Cu2+ was verified by UV-vis spectroscopy, fluorescence analysis, 1H-NMR analysis, mass spectroscopy, DFT study and Job plot analysis. For practical applications, the binding of NCP with OCl? and Cu2+ was determined using a dipstick method and a cell imaging study in a physiological medium using green gram seeds. 2024 The Royal Society of Chemistry.
- Source
- Analytical Methods, Vol-16, No. 5, pp. 676-685.
- Date
- 2023-01-01
- Publisher
- Royal Society of Chemistry
- Coverage
- Kumar M.S., Department of Chemistry, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bangalore, 560029, India; Vishnu S., Department of Chemistry, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bangalore, 560029, India; Dolai M., Department of Chemistry, Prabhat Kumar College, Contai, W.B., Purba Medinipur, 721404, India; Nag A., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bangalore, 560029, India; Bylappa Y., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bangalore, 560029, India; Das A.K., Department of Chemistry, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bangalore, 560029, India
- Rights
- Restricted Access
- Relation
- ISSN: 17599660; PubMed ID: 38189149
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Kumar M.S.; Vishnu S.; Dolai M.; Nag A.; Bylappa Y.; Das A.K., “Viscosity-sensitive and AIE-active bimodal fluorescent probe for the selective detection of OCl? and Cu2+: a dual sensing approach via DFT and biological studies using green gram seeds,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 24, 2025, https://archives.christuniversity.in/items/show/13840.