Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Phytochemical Analysis and Antibacterial Potential of Stevia rebaudiana (Bertoni, 1899) Leaf Extracts against Aeromonas Species: Influence of Extraction Methods and Solvents in Aquaculture Applications Subject The topic of the resource Aeromonas; Anti-inflammatory; Antioxidant; Maceration; Secondary Metabolites; Stevia rebaudiana Description An account of the resource Recent studies have explored Stevia rebaudiana Bertoni leaf extracts for their antibacterial potential and phytochemical content. However, the impact of extraction methods and solvents on aquaculture bacteria remains understudied. This research aimed to evaluate the antibacterial, radical scavenging, and phytochemical properties of S. rebaudiana extracts against Aeromonas species. Dried S. rebaudiana leaves were extracted using methanol (Mt) and ethanol (Et) through Soxhlet and maceration methods (SMt, SEt, MMt and MEt respectively). Soxhlet extraction yielded higher amounts (36.29% for Mt, 23.87% for Et) compared to maceration. Phytochemical analysis identified phenolics, flavonoids, alkaloids, saponin, tannin, and steroids in all extracts. Notably, MEt had elevated phenolic and flavonoid content, while SEt contained more tannins. MEt exhibited the strongest antioxidant activity (IC50 = 67.95g/mL), aligning with its high phenolic and flavonoid levels. In antibacterial assays against Aeromonas strains, ethanol extract showed the largest zone of inhibition (ZOI) of 16.67mm for A. salmonicida, followed by methanol extract (15mm) at 250 mg/mL, using maceration and Soxhlet methods, respectively. However, none of the extracts displayed activity against A. hydrophila. This suggests that cold maceration is a cost-effective method that preserves heat-sensitive secondary metabolites within a shorter extraction time. In conclusion, this study highlights the significance of extraction techniques and solvents in obtaining potent antibacterial and antioxidant extracts from S. rebaudiana leaves. The findings emphasize the potential of these extracts in aquaculture practices and open avenues for further research in utilizing natural compounds for sustainable aquaculture strategies. The Author(s) 2023. Creator An entity primarily responsible for making the resource Raghu A.; Velayudhannair K. Source A related resource from which the described resource is derived Journal of Pure and Applied Microbiology, Vol-17, No. 4, pp. 2352-2366. Publisher An entity responsible for making the resource available Journal of Pure and Applied Microbiology Date A point or period of time associated with an event in the lifecycle of the resource 2023-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.22207/JPAM.17.4.31" target="_blank" rel="noreferrer noopener">https://doi.org/10.22207/JPAM.17.4.31</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85180313687&amp;doi=10.22207%2FJPAM.17.4.31&amp;partnerID=40&amp;md5=65001aec93f33f3406bba6a9d56f2145" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85180313687&amp;doi=10.22207%2fJPAM.17.4.31&amp;partnerID=40&amp;md5=65001aec93f33f3406bba6a9d56f2145</a> Rights Information about rights held in and over the resource All Open Access; Gold Open Access Relation A related resource ISSN: 9737510; LS; 2023-2024; Vol-1; 0812-0826 Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Raghu A., Department of Life Sciences, CHRIST (Deemed to be University), Bangalore Central Campus, Hosur Road, Dharmaram Post, Karnataka, Bengaluru, 560029, India; Velayudhannair K., Department of Life Sciences, CHRIST (Deemed to be University), Bangalore Central Campus, Hosur Road, Dharmaram Post, Karnataka, Bengaluru, 560029, India