Green synthesis, characterization of silver nanoparticles using aqueous leaf extracts of Solanum melongena and in vitro evaluation of antibacterial, pesticidal and anticancer activity in human MDA-MB-231 breast cancer cell lines
- Title
- Green synthesis, characterization of silver nanoparticles using aqueous leaf extracts of Solanum melongena and in vitro evaluation of antibacterial, pesticidal and anticancer activity in human MDA-MB-231 breast cancer cell lines
- Creator
- Pushparaj K.; Balasubramanian B.; Kandasamy Y.; Arumugam V.A.; Kaliannan D.; Arumugam M.; Abdulrahman Alodaini H.; Atef Hatamleh A.; Pappuswamy M.; Meyyazhagan A.
- Description
- The present study validated the green synthesis and characterization of AgNPs using the leaf extracts of Solanum melongena (SM-AgNPs). The efficiency of the phytocompounds in the leaf extract in reducing Ag+ to Ag0 ions was studied. The synthesized SM-AgNPs were characterized by spectroscopic methods and standard methods were adopted for the antioxidant activity, HPTLC, cytotoxicity and antimicrobial assays and pesticidal activity as well. The synthesised nanoparticle was measured to be 20.54 nm which was confirmed by the characterization. The total phenolic content was 5.98 0. GAE/g which indicated potent antioxidant activity. HPTLC profile showed thirteen peaks corresponding to the phenolic compounds with Rf values ranging from 0.05 to 0.95. The average particle size and zeta potential was 75.14 nm-19.8 mV respectively. Cytotoxicity assay of the synthesized AgNP's showed viability percentage of 50.23 at 100 mg/mL. Treatment of MDA-MB-231 cell lines with the SM-AgNPs induced early apoptosis in a greater number of cells. The synthesizedAgNPs were effective against the bacterial isolates Escherichia coli, Pseudomonas aeruginosa, Shigella flexneri, Proteus vulgaris, Klebsiella pneumoniae and Staphylococcus aureus. High mortality of Bemisiatabaci was observed in the100 g/mL of the SM-AgNPs treated concentrations. The study demonstrated the efficiency of SM-AgNPs against cancer cell lines, microbial isolates and insect pests. 2023 The Author(s)
- Source
- Journal of King Saud University - Science, Vol-35, No. 5
- Date
- 2023-01-01
- Publisher
- Elsevier B.V.
- Subject
- Anti-bacterial; Cytotoxicity; Leaves; Pesticidal; Silver nanoparticles; Solanum melongena
- Coverage
- Pushparaj K., Department of Zoology, School of Biosciences, Avinashilingam Institute for Home Science and Higher Education for Women, Tamil Nadu, Coimbatore, 641 043, India; Balasubramanian B., Department of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul, 05006, South Korea; Kandasamy Y., Department of Zoology, School of Biosciences, Avinashilingam Institute for Home Science and Higher Education for Women, Tamil Nadu, Coimbatore, 641 043, India; Arumugam V.A., Department of Human Genetics and Molecular Biology, Bharathiar University, Tamil Nadu, Coimbatore, 641 046, India; Kaliannan D., Department of Infection Biology, School of Medicine, Wonkwang University, lksan, 54538, South Korea; Arumugam M., Department of Botany, School of Life Sciences, Periyar University, Tamil Nadu, Salem, India; Abdulrahman Alodaini H., Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia; Atef Hatamleh A., Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia; Pappuswamy M., Department of Life Sciences, CHRIST (Deemed to be University), 560029 Karnataka, Bengaluru, India; Meyyazhagan A., Department of Life Sciences, CHRIST (Deemed to be University), 560029 Karnataka, Bengaluru, India, Centre of Perinatal and Reproductive Medicine, Department of Translational Medicine and Surgery, University of Perugia, Perugia, Italy
- Rights
- All Open Access; Gold Open Access
- Relation
- ISSN: 10183647
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Pushparaj K.; Balasubramanian B.; Kandasamy Y.; Arumugam V.A.; Kaliannan D.; Arumugam M.; Abdulrahman Alodaini H.; Atef Hatamleh A.; Pappuswamy M.; Meyyazhagan A., “Green synthesis, characterization of silver nanoparticles using aqueous leaf extracts of Solanum melongena and in vitro evaluation of antibacterial, pesticidal and anticancer activity in human MDA-MB-231 breast cancer cell lines,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 26, 2025, https://archives.christuniversity.in/items/show/14182.