Phytochemical Composition, Bioactive Compounds, and Antioxidant Properties of Different Parts of Andrographis macrobotrys Nees
- Title
- Phytochemical Composition, Bioactive Compounds, and Antioxidant Properties of Different Parts of Andrographis macrobotrys Nees
- Creator
- Dalawai D.; Murthy H.N.; Dewir Y.H.; Sebastian J.K.; Nag A.
- Description
- Andrographis macrobotrys Nees is an ethnomedicinal plant belonging to the family Acanthaceae, distributed in the moist deciduous and semi-evergreen forests of the southern Western Ghats of India. The objective of this research was to determine the phytochemical composition and bioactive chemical components using gas chromatography and mass spectrometry (GC-MS) and to check the antioxidant potential of the plant part extracts. A. macrobotrys roots, stems, and leaves were obtained from the species natural habitat in the Western Ghats, India. The bioactive compounds were extracted using a Soxhlet extractor at 5560 C for 8 h in methanol. Identification analysis of A. macrobotrys bioactive compound was performed using GC-MS. Quantitative estimation of phytochemicals was carried out, and the antioxidant capacity of the plant extracts was determined by 2,2?-diphenyl-1-picrylhydrazyl radical scavenging (DPPH) and ferric reducing assays (FRAP). A. macrobotrys has a higher concentration of phenolics in its stem extract than in its root or leaf extracts (124.28 mg and 73.01 mg, respectively), according to spectrophotometric measurements. GC-MS analysis revealed the presence of phytochemicals such as azulene, 2,4-di-tert-butylphenol, benzoic acid, 4-ethoxy-ethyl ester, eicosane, 3-heptadecanol, isopropyl myristate, hexadecanoic acid methyl ester, hexadecanoic acid, 1-butyl-cyclohexanol, 9,12-octadecadienoic acid, alpha-monostearin, and 5-hydroxy-7,8-dimethoxyflavone belonging to various classes of flavonoids, terpenoids, phenolics, fatty acids, and aromatic compounds. Significant bioactive phytochemicals include 2,4-di-tert-butylphenol, 2-methoxy-4-vinylphenol, 5-hydroxy-7,8-dimethoxyflavone, azulene, salvigenin, squalene, and tetrapentacontane. In addition, the antioxidant capability of each of the three extracts was assessed. The stem extract demonstrated impressive DPPH scavenging and ferric reduction activities, with EC50 values of 79 mg/mL and 0.537 0.02 OD at 0.2 mg/mL, respectively. The results demonstrated the importance of A. macrobotrys as a source of medicine and antioxidants. 2023 by the authors.
- Source
- Life, Vol-13, No. 5
- Date
- 2023-01-01
- Publisher
- MDPI
- Subject
- Andrographis; antioxidants; bioactive compounds; GS-MS analysis
- Coverage
- Dalawai D., Department of Botany, Karnatak University, Dharwad, 580003, India; Murthy H.N., Department of Botany, Karnatak University, Dharwad, 580003, India, Department of Horticultural Science, Chungbuk National University, Cheongju, 28644, South Korea; Dewir Y.H., Plant Production Department, College of Food & Agriculture Sciences, King Saud University, Riyadh, 11451, Saudi Arabia; Sebastian J.K., Department of Life Sciences, Christ University, Bangalore, 560029, India; Nag A., Department of Life Sciences, Christ University, Bangalore, 560029, India
- Rights
- All Open Access; Gold Open Access; Green Open Access
- Relation
- ISSN: 20751729
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Dalawai D.; Murthy H.N.; Dewir Y.H.; Sebastian J.K.; Nag A., “Phytochemical Composition, Bioactive Compounds, and Antioxidant Properties of Different Parts of Andrographis macrobotrys Nees,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 25, 2025, https://archives.christuniversity.in/items/show/14296.