Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Optimized production of keratinolytic proteases from Bacillus tropicus LS27 and its application as a sustainable alternative for dehairing, destaining and metal recovery Subject The topic of the resource Bacillus tropicus; Dehairing; Destaining; Keratinase; Metal recovery; Optimization; Protease Description An account of the resource The present study describes the isolation and characterization of Bacillus tropicus LS27 capable of keratinolytic protease production from Russell Market, Shivajinagar, Bangalore, Karnataka, with its diverse application. The ability of this strain to hydrolyze chicken feathers and skim milk was used to assess its keratinolytic and proteolytic properties. The strain identification was done using biochemical and molecular characterization using the 16S rRNA sequencing method. Further a sequential and systematic optimization of the factors affecting the keratinase production was done by initially sorting out the most influential factors (NaCl concentration, pH, inoculum level and incubation period in this study) through one factor at a time approach followed by central composite design based response surface methodology to enhance the keratinase production. Under optimized levels of NaCl (0.55 g/L), pH (7.35), inoculum level (5%) and incubation period (84 h), the keratinase production was enhanced from 41.62 U/mL to 401.67 9.23 U/mL (9.65 fold increase) that corresponds to a feather degradation of 32.67 1.36% was achieved. With regard to the cost effectiveness of application studies, the crude enzyme extracted from the optimized medium was tested for its potential dehairing, destaining and metal recovery properties. Complete dehairing was achieved within 48 h of treatment with crude enzyme without any visible damage to the collagen layer of goat skin. In destaining studies, combination of crude enzyme and detergent solution [1 mL detergent solution (5 mg/mL) and 1 mL crude enzyme] was found to be most effective in removing blood stains from cotton cloth. Silver recovery from used X-ray films was achieved within 6 min of treatment with crude enzyme maintained at 40 C. 2023 Elsevier Inc. Creator An entity primarily responsible for making the resource Liya S.M.; Umesh M.; Nag A.; Chinnathambi A.; Alharbi S.A.; Jhanani G.K.; Shanmugam S.; Brindhadevi K. Source A related resource from which the described resource is derived Environmental Research, Vol-221 Publisher An entity responsible for making the resource available Academic Press Inc. Date A point or period of time associated with an event in the lifecycle of the resource 2023-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1016/j.envres.2023.115283" target="_blank" rel="noreferrer noopener">https://doi.org/10.1016/j.envres.2023.115283</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85146445028&amp;doi=10.1016%2Fj.envres.2023.115283&amp;partnerID=40&amp;md5=62e555f71d486d4ce092b92bc30f1894" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85146445028&amp;doi=10.1016%2fj.envres.2023.115283&amp;partnerID=40&amp;md5=62e555f71d486d4ce092b92bc30f1894</a> Rights Information about rights held in and over the resource Restricted Access Relation A related resource ISSN: 139351; PubMed ID: 36639016; CODEN: ENVRA Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Liya S.M., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bangalore, India; Umesh M., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bangalore, India; Nag A., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bangalore, India; Chinnathambi A., Department of Botany and Microbiology, College of Science, King Saud University, PO Box -2455, Riyadh, 11451, Saudi Arabia; Alharbi S.A., Department of Botany and Microbiology, College of Science, King Saud University, PO Box -2455, Riyadh, 11451, Saudi Arabia; Jhanani G.K., Center for Transdisciplinary Research (CFTR), Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India; Shanmugam S., Chair of Biosystems Engineering, Institute of Forestry and Engineering, Estonian University of Life Sciences, Kreutzwaldi 56, Tartu, 51014, Estonia; Brindhadevi K., University Centre for Research &amp; Development, Department of Chemistry, Chandigarh University, Mohali, India