Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Production of Boeravinone-B, total phenolic, flavonoid content and antioxidant activity from callus cultures of Punarnava (BoerhaviadiffusaL.) Subject The topic of the resource Boeravinone-B; Boerhavia diffusa; callus cultures; HPLC; Punarnava; rotenoid Description An account of the resource Boerhavia diffusa L. (Punarnava) is a medicinal herb, rich in diversified plant secondary metabolites used in curing various health ailments. Boeravinone-B is one of the important phytochemicals reported in Punarnava, possessing various pharmacological activities. It belongs to the family of rotenoids, belonging to the isoflavone group. Production of Boeravinone-B from the Punarnava through conventional propagation is comparatively very low, and alternative interventions are of utmost importance to meet the growing demand. In view of this, the present study aims to develop biotechnological approaches like cell/tissue culture as a substitute strategy for the accumulation of biomass and Boeravinone-B biosynthesis. Callus was established from leaf explants of Boerhavia diffusa L. when cultured on MS semi solid medium fortified with varied concentrations and combinations of auxins and cytokinins. The callus induced on Murashige and Skoog medium (MS medium) supplemented with 5.0 ppm 2,4-Dichlorophenoxyacetic acid (2,4-D) favored the highest production of Boeravinone-B analyzed through High-performance Liquid chromatography (HPLC) and it was found to be 673.95 ?g g-1 Dry weight (DW). The total phenolic and flavonoid content were determined for the callus extracts and the results showed that callus induced on 5.0 ppm 2,4-D medium showed the highest phenolic and flavonoid content, which was 63.48 mg g-1 Gallic acid equivalent (GAE) Dry weight (DW), and 30.22 mg g-1 Quercetin equivalent (QE) DW. Similarly, antioxidant activities (radical scavenging, metal chelating, and reducing power) were performed, and it was found that callus induced on 5.0 ppm 2,4-D showed the highest anti-oxidant potential. Radical scavenging activity was found to be 91.1%, and 74% of metal chelating activity was recorded, and a similar trend was observed with respect to reducing power as well. The results of the present study lay foundation for optimization and subsequent large-scale production of Boeravinone-B from callus/cell suspension cultures. The Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/ by/4.0/) Creator An entity primarily responsible for making the resource Sudheer W.N.; Nagella P. Source A related resource from which the described resource is derived Plant Science Today, No. 10 Publisher An entity responsible for making the resource available Horizon e-Publishing Group Date A point or period of time associated with an event in the lifecycle of the resource 2023-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.14719/pst.2212" target="_blank" rel="noreferrer noopener">https://doi.org/10.14719/pst.2212</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85151429875&amp;doi=10.14719%2Fpst.2212&amp;partnerID=40&amp;md5=cdb7a5d5be483813f536082839b84d75" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85151429875&amp;doi=10.14719%2fpst.2212&amp;partnerID=40&amp;md5=cdb7a5d5be483813f536082839b84d75</a> Rights Information about rights held in and over the resource All Open Access; Gold Open Access; Green Open Access Relation A related resource ISSN: 23481900 Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Sudheer W.N., Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, 560029, India; Nagella P., Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, 560029, India