Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Sesquiterpenoid-rich Java Ginger rhizome extract prompts autophagic cell death in cervical cancer cell SiHa mainly by modulating cellular redox homeostasis Subject The topic of the resource Cancer biology; Cell culture; Cytotoxicity; Flow cytometry; Western blot analysis Description An account of the resource Java Ginger or Curcuma zanthorrhiza Roxb. has long gained focus among tribal people of Java, for its medicinal properties mainly against gynaecological challenges. The present study aims to identify the most potent phytocompound present in the extract and determine primary mode of action accountable for cytotoxic activity of Curcuma zanthorrhiza rhizome extract against HPV16-positive SiHa cervical cancer cells. The phytochemically-rich extract of rhizome (CZM) was capable to inhibit proliferation of target cells in a dose-dependent manner with an IC50 of 150?g/ml. Dysregulation of intercellular antioxidant defence system resulted to surges in ROS and RNS level, increased calcium concentration and compromised mitochondrial membrane potential. Nucleus got affected, cell cycle dynamics got impaired while clonogenicity and migration ability diminished. Expression of viral oncogenes E7 and E6 decreased significantly. Accumulation of toxic cell metabolite and decrease in level of essential ones continued. Finally, alteration in PI3K/AKT/mTOR signalling route was followed by onset of autophagic cell death concomitant with the upregulated expression of Beclin1, Atg5-12 and LC3II. Curcumin and a novel crystal as well as few phyto-fractions were isolated by column chromatography. Of these, curcumin was found to be most potent in inducing cytotoxicity in SiHa while two other fractions also showed significant activity. Thus, CZM acted against SiHa cells by inducing autophagy that commences in compliance to the changes in PI3K/AKT/mTOR pathway mainly in response to oxidative stress. To the best of our knowledge this is the first report of Curcuma zanthorrhiza Roxb. inducing autophagy. 2022, King Abdulaziz City for Science and Technology. Creator An entity primarily responsible for making the resource Nath S.; Patra D.; Nag A.; Kundu R. Source A related resource from which the described resource is derived 3 Biotech, Vol-13, No. 1 Publisher An entity responsible for making the resource available Springer Science and Business Media Deutschland GmbH Date A point or period of time associated with an event in the lifecycle of the resource 2023-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1007/s13205-022-03415-9" target="_blank" rel="noreferrer noopener">https://doi.org/10.1007/s13205-022-03415-9</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85144292287&amp;doi=10.1007%2Fs13205-022-03415-9&amp;partnerID=40&amp;md5=5221685ca3a2c97f43632d7a4cf47c28" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85144292287&amp;doi=10.1007%2fs13205-022-03415-9&amp;partnerID=40&amp;md5=5221685ca3a2c97f43632d7a4cf47c28</a> Rights Information about rights held in and over the resource All Open Access; Green Open Access Relation A related resource ISSN: 2190572X Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Nath S., Centre of Advanced Study, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, India; Patra D., Department of Chemistry, Taki Government College, West Bengal, Taki, 743426, India; Nag A., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560029, India; Kundu R., Centre of Advanced Study, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, India