Biocontrol of Aedes aegypti using Talaromyces islandicus Synthesized Silver Nanoparticles
Aedes aegypti; Antilarval; Antioxidant; Silver nanoparticles; Talaromyces islandicus
Aedes aegypti is the vector that spreads the dengue virus, causing dengue fever and dengue hemorrhagic fever. With more than half the worlds population at the risk of acquiring this infection, controlling the Aedes mosquitoes is the only path to limit the spread of the fatal disease. The emergence of insect resistance in mosquitoes raised the need for developing novel insecticides. Present research is focused on using fungus (Talaromyces islandicus) as the biosystem in the synthesis of nanoparticles. Myco-synthesized silver nanoparticles were characterized using UV-visible spectrometry that exhibited a peak at 429 nm. The XRD spectral peaks were in the range of 27.83, 32.27, 38.23and 65.01. The FTIR spectrum showed peaks corresponding to O-H, N-O, S=O, etc. representing the silver nanoparticles. SEM and EDAX represent the formation of silver ions that are spherical in shape with a size range of 23 to 26 nm. The antioxidant activity of silver nanoparticles and the extract of Talaromyces islandicus were assessed by DPPH assay, reducing power assay and hydrogen peroxide assay. The nanoparticles studied for its bio efficacy against the larval stages of Aedes aegypti indicated the LC50 value of 352.03, 389.86, 397.72 and 443.50 when tested against first, second, third and fourth instar larvae. respectively. The LC50 value of 540.41 was determined against the pupae of Aedes. The predatory efficiency of P. reticulata indicated the positive feeding behaviour of the fish when exposed to the silver nanoparticles. The cell toxicity assay was conducted against C6/36 insect cell lines and the cell viability inhibition was calculated. A toxic free, environmentally acceptable approach for controlling the mosquito vector by utilizing fungal nanoparticles was assessed and their efficacy in vector control was analyzed in this study. 2022 Chemical Publishing Co.. All rights reserved.
George J.A.; Vinanthi Rajalakshmi K.S.; Rajendran R.; Paari K.A.
Asian Journal of Chemistry, Vol-34, No. 11, pp. 2897-2904.
https://doi.org/10.14233/ajchem.2022.23908 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140929996&doi=10.14233%2fajchem.2022.23908&partnerID=40&md5=edff48d23670b46109d6f0a2763041fd
George J.A., Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, 560029, India; Vinanthi Rajalakshmi K.S., Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, 560029, India; Rajendran R., Department of Energy and Environment Engineering, CSIR-Indian Institute of Chemical Technology, Hyderabad, 500007, India; Paari K.A., Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, 560029, India