Biotic elicitation mediated in vitro production of L-DOPA from Mucuna pruriens (L.) DC. cell cultures

Title

Biotic elicitation mediated in vitro production of L-DOPA from Mucuna pruriens (L.) DC. cell cultures

Creator

Rakesh B.; Praveen N.

Description

With the emerging rise in the need for drugs extracted from various plant sources, there also arises the need for the optimum production of the drugs on a larger scale and conservation of those medicinal plants using different in vitro techniques and biotechnological approaches. Plant tissue culture techniques play a prominent role in mass multiplication of the plant. Whereas, strategies such as precursor feeding, elicitation, increases the metabolite content several-fold. Thus, an attempt of using the biotic elicitors for enhancing L-DOPA production, the anti-Parkinsons drug from Mucuna pruriens (L.) DC. cell cultures, has been reported in the present study. Aqueous extracts of algae [Amphiroa anceps (AA), Gracillaria ferogusonii (GF), Kappaphycus striatum (KS), and Sargassum lanceolatum (SL)], fungus [Aspergillus sps. (AS), Penicillium sps. (PE), and Cordyceps sps (CO)], and polysaccharide [Chitosan (CH)] solution were exposed to the cell cultures for 3, 6, and 9 d, respectively, and their effect on biomass and L-DOPA production was noted. This is the first report demonstrating the enhancement of biomass and L-DOPA from M. pruriens cell cultures with the use of various algal and fungal elicitors. Based on productivity (L-DOPA concentration biomass volume), it was observed that Cordyceps showed the best result and enhanced both biomass and metabolite to a greater scale. The elicitors, which showed a significant increase, are as follows: CO > AS > PE > CH > AA > KS > GF > SL. On the whole, it was noted that fungal extracts showed better results than algae. 2022, The Society for In Vitro Biology.

Source

In Vitro Cellular and Developmental Biology - Plant, Vol-58, No. 6, pp. 1077-1089.

Date

2022-01-01

Publisher

Springer

Subject

Anti-Parkinsons; Biotic elicitors; In vitro elicitation; L-DOPA; Mucuna pruriens

Coverage

Rakesh B., Department of Life Sciences, CHRIST (Deemed To Be University), Hosur Road, Bengaluru, 560 029, India; Praveen N., Department of Life Sciences, CHRIST (Deemed To Be University), Hosur Road, Bengaluru, 560 029, India

Rights

Restricted Access

Relation

ISSN: 10545476; CODEN: IVCPE

Format

Online

Language

English

Type

Article

Identifier

https://doi.org/10.1007/s11627-022-10303-7

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140003403&doi=10.1007%2fs11627-022-10303-7&partnerID=40&md5=2f44304a2863246c1acbfadbe1efff51