Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Structural and functional characterization of a hypothetical protein in the RD7 region in clinical isolates of Mycobacterium tuberculosis an in silico approach to candidate vaccines Subject The topic of the resource Flavodoxin-like fold; Immunogenic; M. canetti; Membrane protein; Mycobacterium tuberculosis; PSPIRED; Ramachandran plot; RD7; Robetta; Vaccine Description An account of the resource Background: Mycobacterium tuberculosis has been ravaging humans by inflicting respiratory tuberculosis since centuries. Bacillus Calmette Guerine (BCG) is the only vaccine available for tuberculosis, and it is known to be poorly effective against adult tuberculosis. Proteins belonging to the ESAT-6 family and PE/PPE family show immune responses and are included in different vaccine trials. Herein, we study the functional and structural characterization of a 248 amino acid long putative protein novel hypothetical protein 1 (NHP1) present in the RD7 region of Mycobacterium tuberculosis (identified first by subtractive hybridization in the clinical isolate RGTB123) using bioinformatics tools. Results: Physicochemical properties were studied using Expasy ProtParam and SMS software. We predicted different B-cell and T-cell epitopes by using the immune epitope database (IEDB) and also tested antigenicity, immunogenicity, and allergenicity. Secondary structure of the protein predicted 30% alpha helices, 20% beta strands, and 48% random coils. Tertiary structure of the protein was predicted using the Robetta server using the Mycobacterium smegmatis protein as the putative protein with homology. Structural evaluations were done with Ramachandran plot analysis, ProSA-web, and VERIFY3D, and with GalaxyWEB server, a more stable structure was validated with good stereo chemical properties. Conclusion: The present study of a subtracted genomic locus using various bioinformatics tools indicated good immunological properties of the putative mycobacterial protein, NHP1. Evidence obtained from the analyses of NHP1 using structure prediction tools strongly point to the fact that NHP1 is an ancient protein having flavodoxin folding structure with ATP binding sites. Positive scores were obtained for antigenicity, immunogenicity, and virulence too, implying the possibility of NHP1 to be a potential vaccine candidate. Such computational studies might give clues for developing newer vaccines for tuberculosis, which is the need of the hour. 2022, The Author(s). Creator An entity primarily responsible for making the resource Kootery K.P.; Sarojini S. Source A related resource from which the described resource is derived Journal of Genetic Engineering and Biotechnology, Vol-20, No. 1 Publisher An entity responsible for making the resource available Springer Science and Business Media Deutschland GmbH Date A point or period of time associated with an event in the lifecycle of the resource 2022-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1186/s43141-022-00340-5" target="_blank" rel="noreferrer noopener">https://doi.org/10.1186/s43141-022-00340-5</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85128212527&amp;doi=10.1186%2Fs43141-022-00340-5&amp;partnerID=40&amp;md5=a4d12da7dd2e715489b12b08e532da7f" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85128212527&amp;doi=10.1186%2fs43141-022-00340-5&amp;partnerID=40&amp;md5=a4d12da7dd2e715489b12b08e532da7f</a> Rights Information about rights held in and over the resource All Open Access; Gold Open Access; Green Open Access Relation A related resource ISSN: 1687157X Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Kootery K.P., Department of Lifesciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, 560029, India; Sarojini S., Department of Lifesciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, 560029, India