Antibacterial activity and spectroscopic characteristics of silver nanoparticles synthesized via plant and invitro leaf-derived callus extracts of Mucuna pruriens (L.) DC
- Title
- Antibacterial activity and spectroscopic characteristics of silver nanoparticles synthesized via plant and invitro leaf-derived callus extracts of Mucuna pruriens (L.) DC
- Creator
- B R.; N S.; K J R.K.; A M.; M R S.K.; N P.
- Description
- In this paper, we describe the green synthesis of silver nanoparticles (AgNPs) using plant extracts, as well as their structural, optical, and antibacterial activities against Escherichia coli and Bacillus velezensis bacteria. For the study, several sections (root, leaves, and seed) of Mucuna pruriens, an immense medicinal herbal used to treat Parkinson's disease, were evaluated. Also, the seeds were grown in-vitro in the modified Murashige and Skoog's medium and the leaf derived callus supplemented by different phytohormones like picloram (pic), thidiazuron (TDZ), 6-benzylaminopurine (BAP) and 2-isopentenyl adenine (2-iP) were selected for this study. This is the first report of distinct callus obtained from M. pruriens and used for green nanoparticle synthesis. The prepared materials were thoroughly evaluated for structural and optical properties using XRD, FTIR, and UV-Visible spectroscopy. During synthesis, the colour changes from colourless to reddish brown, and the existence of an SPR peak in the absorption spectra confirms the formation of AgNPs. The FTIR spectra reveals the presence of phenolic group and alkyl ether groups, which were responsible for the reduction of silver ions during the green synthesis. The aqueous extracts of plant parts and callus obtained from different hormones showed very poor antibacterial activity. However, the green synthesized nanoparticles fabricated by different plant extracts showed good antimicrobial activity towards E. coli and B. velezensis bacteria. amongst the investigated results, highest antimicrobial activity against E. coli (84.8%) and B. velezensis (78.1%) was shown by AgNPs mediated by leaf extracts respectively followed by 0.5 mg/L pic and 2.0 mg/L BAP callus mediated silver nanoparticle solution. According to the findings, green produced AgNPs are promising candidates for antibacterial applications against E. coli and B. velezensis. 2022 SAAB
- Source
- South African Journal of Botany, Vol-148, pp. 251-258.
- Date
- 2022-01-01
- Publisher
- Elsevier B.V.
- Subject
- Green synthesis; In-vitro callus; Mucuna pruriens; Silver nanoparticles; Surface plasmon resonance
- Coverage
- B R., Department of Life Sciences, Christ (Deemed to be University), Hosur Road, Bengaluru, 560 029, India; N S., Department of Physics, RV Institute of Technology and Management, Bengaluru, 560 076, India; K J R.K., Department of Chemistry, RV Institute of Technology and Management, Bengaluru, 560 076, India; A M., Department of Physics, Dayananda Sagar College of Engineering, Kumaraswamy Layout, Karnataka, Bangalore, 560 078, India; M R S.K., Department of Physics, RV Institute of Technology and Management, Bengaluru, 560 076, India; N P., Department of Life Sciences, Christ (Deemed to be University), Hosur Road, Bengaluru, 560 029, India
- Rights
- Restricted Access
- Relation
- ISSN: 2546299; CODEN: SAJBD
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
B R.; N S.; K J R.K.; A M.; M R S.K.; N P., “Antibacterial activity and spectroscopic characteristics of silver nanoparticles synthesized via plant and invitro leaf-derived callus extracts of Mucuna pruriens (L.) DC,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 27, 2025, https://archives.christuniversity.in/items/show/14977.