Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource PA1 cells containing a truncated DNA polymerase ? protein are more sensitive to gamma radiation Subject The topic of the resource DNA polymerase ?; DNA repair; Molecular docking simulation; Ovarian epithelial carcinoma; Radiotherapy Description An account of the resource Purpose: DNA polymerase ? (Pol?) acts in the base excision repair (BER) pathway. Mutations in DNA polymerase ? (Pol?) are associated with different cancers. A variant of Pol? with a 97 amino acid de-letion (Pol??), in heterozygous conditions with wild-type Pol?, was identified in sporadic ovarian tumor samples. This study aims to evaluate the gamma radiation sensitivity of Pol?? for possible target therapy in ovarian cancer treatment. Materials and Methods: Pol?? cDNA was cloned in a GFP vector and transfected in PA1 cells. Stable cells (PA1Pol??) were treated with60Co sourced gamma-ray (015 Gy) to investigate their radiation sensitivity. The affinity of Pol?? with DNA evaluated by DNA protein in silico docking experiments. Results: The result showed a statistically significant (p &lt; 0.05) higher sensitivity towards radiation at different doses (015 Gy) and time-point (4872 hours) for PA1Pol?? cells in comparison with nor-mal PA1 cells. Ten Gy of gamma radiation was found to be the optimal dose. Significantly more PA-1Pol?? cells were killed at this dose than PA1 cells after 48 hours of treatment via an apoptotic pathway. The in silico docking experiments revealed that Pol?? has more substantial binding potential towards the dsDNA than wild-type Pol?, suggesting a possible failure of BER pathway that results in cell death. Conclusion: Our study showed that the PA1Pol?? cells were more susceptible than PA1 cells to gamma radiation. In the future, the potentiality of ionizing radiation to treat this type of cancer will be checked in animal models. 2022 The Korean Society for Radiation Oncology. Creator An entity primarily responsible for making the resource Patra A.; Nag A.; Chakraborty A.; Bhattacharyya N. Source A related resource from which the described resource is derived Radiation Oncology Journal, Vol-40, No. 1, pp. 66-78. Publisher An entity responsible for making the resource available Department of Radiation Oncology Date A point or period of time associated with an event in the lifecycle of the resource 2022-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.3857/roj.2021.00689" target="_blank" rel="noreferrer noopener">https://doi.org/10.3857/roj.2021.00689</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85128481705&amp;doi=10.3857%2Froj.2021.00689&amp;partnerID=40&amp;md5=9371dc5e120fbaa914bb2f931a7ed0f9" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85128481705&amp;doi=10.3857%2froj.2021.00689&amp;partnerID=40&amp;md5=9371dc5e120fbaa914bb2f931a7ed0f9</a> Rights Information about rights held in and over the resource All Open Access; Gold Open Access; Green Open Access Relation A related resource ISSN: 22341900 Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Patra A., Department of Biotechnology, Panskura Banamali College, West Bengal, India; Nag A., Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, India; Chakraborty A., UGC DAE, Consortium for Scientific Research, Kolkata Centre, Kolkata, India; Bhattacharyya N., Department of Biotechnology, Panskura Banamali College, West Bengal, India