Distillery effluent valorization through cost effective production of polyhydroxyalkanoate: optimization and characterization
Bacillus subtilis NCDC 0671; Distillery effluent; FTIR; Polyhydroxyalkanoate; Response surface methodology
The devastating effect of fossil plastics in the biosphere has tuned the concern for bioplastic production in the last few decades. Polyhydroxyalkanoate, a biopolyester, has a wide range of applications as they impose positive societal impact by being biodegradable and void of any ill-effects when used in vivo. Despite their eco-friendly nature, the outreach of PHA is bounded in industrial scale as the overall expense is highly comparable to conventional plastics. Therefore, in an attempt to attain a feasible production, the present study aims at utilizing raw distillery effluent for PHA production using Bacillus subtilis NCDC 0671. Different dilutions of spent wash (5%, 10%, 15%, and 20%) were assessed for PHA production in the modified medium among which 10% showed maximum PHA accumulation. Furthermore, statistical optimization by response surface methodology enhanced PHA synthesis to 6.3g/L which is 3.3-fold increases. FTIR and NMR characterization of the biopolymer from the optimized medium was similar to the previous literature which provides a promising approach for cost effective production. 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Springer Science and Business Media Deutschland GmbH
Priyanka K., Department of Microbial Biotechnology, Bharathiar University, Tamil Nadu, Coimbatore, 641046, India; Umesh M., Department of Life Sciences, CHRIST (Deemed to Be University), Hosur Road, Karnataka, Bengaluru, 560029, India; Preethi K., Department of Microbial Biotechnology, Bharathiar University, Tamil Nadu, Coimbatore, 641046, India