Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource A sustainable approach for fish waste valorization through polyhydroxyalkanoate production by Bacillus megaterium NCDC0679 and its optimization studies Subject The topic of the resource Bacillus megaterium; BoxBehnken design; Fish scale hydrolysate; PHA; PlackettBurman design; Response surface methodology Description An account of the resource Polyhydroxyalkanoates (PHAs) are considered as the only class of truly biodegradable and biocompatible polymers. Although extensive research has been carried out in producing them from a wide variety of organisms, their commercialization still faces hurdles majorly associated with the cost of production media. This research work exploits the use of discarded fish scale waste as a major media component for biopolymer production. The major novelty of the research work is the utilization of a Bacillus megaterium NCDC0679 for PHA production using fish scale waste that is not reported previously. Furthermore, a sequential and systematic statistical optimization strategy employing response surface methodology was used to trace out the level of the most significant variables and their interaction effects on PHA production add to the significant novelty of this work. The significance of the model developed was determined from the p values of ANOVA. Under optimized levels of glucose (50g/L), NaCl (0.125g/L), and fish scale hydrolysate concentration (62.5% v/v), maximum PHA yield of 6.33g/L was achieved in the shake flask culture system. This was found to be 5.50-fold higher than the unoptimized medium. The ANOVA results established the significance of the model (p &lt; 0.05). The extracted polymer was characterized through Fourier-transform infrared (FTIR), nuclear magnetic resonance (NMR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). Thus, the present investigation suggests an innovative method for valorization of fish scale waste for commercial production of PHA. 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. Creator An entity primarily responsible for making the resource Umesh M.; Suresh S.; Sarojini S.; Santosh A.S. Source A related resource from which the described resource is derived Biomass Conversion and Biorefinery Publisher An entity responsible for making the resource available Springer Science and Business Media Deutschland GmbH Date A point or period of time associated with an event in the lifecycle of the resource 2022-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1007/s13399-022-03360-w" target="_blank" rel="noreferrer noopener">https://doi.org/10.1007/s13399-022-03360-w</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140085031&amp;doi=10.1007%2Fs13399-022-03360-w&amp;partnerID=40&amp;md5=2ab93c4840979c9340f2e9fed5562345" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140085031&amp;doi=10.1007%2fs13399-022-03360-w&amp;partnerID=40&amp;md5=2ab93c4840979c9340f2e9fed5562345</a> Rights Information about rights held in and over the resource Restricted Access Relation A related resource ISSN: 21906815 Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Umesh M., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bengaluru, 560029, India; Suresh S., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bengaluru, 560029, India; Sarojini S., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bengaluru, 560029, India; Santosh A.S., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bengaluru, 560029, India