Bacterial diversity of geochemically distinct hot springs located in Maharashtra, India
Bacterial diversity; Geomicrobiology; High-throughput sequencing; Hot springs
Bacterial diversity of four thermally different hot springs of Ratnagiri district, Maharashtra, India, was investigated using culture-dependent and culture-independent approaches. A total of 144 bacterial cultures were isolated and identified using MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry) and 16S rRNA gene sequencing. Culture-independent analysis by Ion Torrent sequencing targeting the V3 region of the 16S rRNA gene revealed the predominance of Firmicutes across all the hot springs, followed by Chloroflexi, Bacteroidetes, Cyanobacteria, Proteobacteria, Armatimonadetes, Actinobacteria, Nitrospirae, Acidobacteria, and DeinococcusThermus, with subtle differences in their abundance. At the lower taxonomic rank of genus, we noted the prevalence of Acinetobacter followed by Clostridium, Planomicrobium, Bacillus, Streptomyces, and Leptolyngbya. Metagenomics imputation using in silico approach revealed divergence in the metabolic capabilities of bacterial communities along the thermal gradient of host springs, with site TS (63C) featuring the abundant functional gene families. 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Mathan Kumar R.; Jani K.; Parvathi J.R.; Thomas B.M.; Raja S.S.S.; Pandey A.; Sharma A.
Archives of Microbiology, Vol-204, No. 1
Springer Science and Business Media Deutschland GmbH
Mathan Kumar R., Government College of Arts and Science, Kurumbalur, Perambalur, (Formerly, Bharathidasan University Constituent College, Perambalur), Tamil Nadu, Kurumbalur, 621212, India; Jani K., DBT-National Centre for Cell Science, Pune, 411007, India; Parvathi J.R., Somaiya Institute for Research and Consultancy (SIRAC), Somaiya Vidyavihar University, Mumbai, 400077, India; Thomas B.M., Somaiya Institute for Research and Consultancy (SIRAC), Somaiya Vidyavihar University, Mumbai, 400077, India, CHRIST (Deemed to be University), Pune, 412112, India; Raja S.S.S., Government College of Arts and Science, Kurumbalur, Perambalur, (Formerly, Bharathidasan University Constituent College, Perambalur), Tamil Nadu, Kurumbalur, 621212, India; Pandey A., Department of Biotechnology, Graphic Era (Deemed to be University), Dehra Dun, 248002, India; Sharma A., DBT-National Centre for Cell Science, Pune, 411007, India