Eggshells biowaste for hydroxyapatite green synthesis using extract piper betel leaf - Evaluation of antibacterial and antibiofilm activity
- Title
- Eggshells biowaste for hydroxyapatite green synthesis using extract piper betel leaf - Evaluation of antibacterial and antibiofilm activity
- Creator
- Umesh M.; Choudhury D.D.; Shanmugam S.; Ganesan S.; Alsehli M.; Elfasakhany A.; Pugazhendhi A.
- Description
- The present research work reports the biosynthesis of hydroxyapatite (HAp) from eggshells and green synthesis of HAp from eggshells with incorporation of Piper betel leaf extract (PBL-HAp) using microwave conversion method. Although there are several works on synthesis of HAp from eggshells and other calcium and phosphorus rich substrates, the incorporation of herbal extract with HAp to promote antimicrobial and antibiofilm activity is less explored and reported. This research work highlights a simple and cost-effective method for development of antimicrobial biomaterials by combining the concepts of waste management, biomaterial science, and herbal medicine. In the present study, characterization of synthesized HAp was applied by X-ray Diffraction (XRD), Fourier Transform Infrared (FTIR) spectroscopy, Proton Nuclear Magnetic Resonance (1H NMR) spectroscopy, and morphological analysis using Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The characterization results indicated that the prepared HAp and PBL-HAp were pure b-type carbonated HAp. The PBL-HAp was checked for its antibacterial activity using the well diffusion method and biofilm inhibitory activity by crystal violet assay against some common pathogens. The antibacterial activities against Staphylococcus aureus and biofilm inhibitory activities against Escherichia coli, Vibrio harveyi, Pseudomonas aeruginosa, and Staphylococcus aureus of Piper betel leaf extract coated HAp (PBL-HAp) were showed to be significant and offered a promising role for the development of potent dental biomaterials. 2021 Elsevier Inc.
- Source
- Environmental Research, Vol-200
- Date
- 2021-01-01
- Publisher
- Academic Press Inc.
- Subject
- Antibacterial property; Antibiofilm activity; HAp composites; Hydroxyapatite; Piper betel leaf
- Coverage
- Umesh M., Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, Karnataka, India; Choudhury D.D., Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, Karnataka, India; Shanmugam S., Bioprocess Laboratory, Department of Microbial Biotechnology, Bharathiar University, Coimbatore, 641046, India; Ganesan S., Department of Safety, Health and Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, 807, Taiwan; Alsehli M., Mechanical Engineering Department, College of Engineering, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia; Elfasakhany A., Mechanical Engineering Department, College of Engineering, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia; Pugazhendhi A., School of Renewable Energy, Maejo University, Chiang Mai, 50290, Thailand, College of Medical and Health Science, Asia University, Taichung, Taiwan
- Rights
- Restricted Access
- Relation
- ISSN: 139351; PubMed ID: 34129868; CODEN: ENVRA
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Umesh M.; Choudhury D.D.; Shanmugam S.; Ganesan S.; Alsehli M.; Elfasakhany A.; Pugazhendhi A., “Eggshells biowaste for hydroxyapatite green synthesis using extract piper betel leaf - Evaluation of antibacterial and antibiofilm activity,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 24, 2025, https://archives.christuniversity.in/items/show/15658.