Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Bromelain enhances digestibility of Spirulina-based fish feed Subject The topic of the resource Arthrospira platensis; In vitro digestibility; Microalgae; Oreochromis mossambicus; Papain; Trypsin Description An account of the resource Microalgae like Spirulina (Arthrospira platensis) are protein rich and can be alternative protein sources to fishmeal and soybean meal in fish feed formulation. The present study aims to improve the protein bioavailability of Spirulina by cost-effective protein extraction followed by protease supplementation in fish feed, using in vitro studies. Different extraction procedures such as microwave-assisted, high pressure, and temperature-mediated extraction, boiling and an isoelectric precipitation were employed to study the protein yield from Spirulina powder, and this was compared with the conventional soybean meal and fishmeal conditioning during feed manufacture. Bromelain is a potent protease that has not been widely used as a feed additive with Spirulina. To study the comparative efficiency of bromelain and other proteases like papain and trypsin on Spirulina and conventional feed substrates, a protease assay was performed at different temperatures and enzyme concentrations. The digestibility of these substrates was also studied in vitro, using gut extracts from the fingerlings of Mozambique tilapia (Oreochromis mossambicus). Unlike an in vivo feeding trial, a novel method was used to study the effect of protease supplementation on the inherent digestibility of the gut with an in vitro method. Bromelain showed the highest activity on all the substrates at both the temperatures. Bromelain supplementation improved the in vitro digestibility of the Spirulina that were subjected to protein extraction, more than the un-extracted one. The results of the present in vitro study suggest that Spirulina could serve as an alternative protein source, and bromelain-based supplementation could improve the digestibility of Spirulina-based fish diets. 2021, Springer Nature B.V. Creator An entity primarily responsible for making the resource Sharma S.A.; Surveswaran S.; Arulraj J.; Velayudhannair K. Source A related resource from which the described resource is derived Journal of Applied Phycology, Vol-33, No. 2, pp. 967-977. Publisher An entity responsible for making the resource available Springer Science and Business Media B.V. Date A point or period of time associated with an event in the lifecycle of the resource 2021-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1007/s10811-020-02337-4" target="_blank" rel="noreferrer noopener">https://doi.org/10.1007/s10811-020-02337-4</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099092228&amp;doi=10.1007%2Fs10811-020-02337-4&amp;partnerID=40&amp;md5=edc22a7527f9617fc3d6193407646d2c" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099092228&amp;doi=10.1007%2fs10811-020-02337-4&amp;partnerID=40&amp;md5=edc22a7527f9617fc3d6193407646d2c</a> Rights Information about rights held in and over the resource Restricted Access Relation A related resource ISSN: 9218971; CODEN: JAPPE Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Sharma S.A., Department of Life Sciences, CHRIST (Deemed to be University), Bangalore Central Campus, Hosur Road, Bengaluru, 560029, Karnataka, India; Surveswaran S., Department of Life Sciences, CHRIST (Deemed to be University), Bangalore Central Campus, Hosur Road, Bengaluru, 560029, Karnataka, India; Arulraj J., Department of Chemistry, CHRIST (Deemed to be University), Bangalore Central Campus, Hosur Road, Bengaluru, 560029, Karnataka, India; Velayudhannair K., Department of Life Sciences, CHRIST (Deemed to be University), Bangalore Central Campus, Hosur Road, Bengaluru, 560029, Karnataka, India