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              <text>Structure and biological properties of exopolysaccharide isolated from Citrobacter freundii</text>
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              <text>Antioxidant activity; Citrobacter freundii; Cytotoxicity; Exopolysaccharide; NMR studies; Structure</text>
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              <text>This study aimed to investigate the molecular characterization, antioxidant activity in vitro, cytotoxicity study of an exopolysaccharide isolated from Citrobacter freundii. Firstly, the culture conditions were standardized by the Design of experiments (DoE) based approach, and the final yield of thecrude exopolysaccharide was optimized at 2568  169 mg L?1. One large fraction of exopolysaccharide was obtained from the culture filtrate by size exclusion chromatography and molecular characteristics were studied. A new mannose rich exopolysaccharide (Fraction-I) with average molecular weight ~ 1.34 105 Da was isolated. The sugar analysis showed the presence of mannose and glucose in a molar ratio of nearly 7:2 respectively. The structure of the repeating unit in the exopolysaccharide was determined through chemical and 1D/2D- NMR experiments as:[Formula presented] Finally, the antioxidant activity, and the cytotoxicity of the exopolysaccharide were investigated and the relationship with molecular properties was discussed as well.  2020 Elsevier B.V.</text>
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              <text>Choudhuri I.; Khanra K.; Maity P.; Patra A.; Maity G.N.; Pati B.R.; Nag A.; Mondal S.; Bhattacharyya N.</text>
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              <text>International Journal of Biological Macromolecules, Vol-168, pp. 537-549.</text>
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              <text>Elsevier B.V.</text>
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              <text>2021-01-01</text>
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              <text>&lt;a href="https://doi.org/10.1016/j.ijbiomac.2020.12.063" target="_blank" rel="noreferrer noopener"&gt;https://doi.org/10.1016/j.ijbiomac.2020.12.063&lt;/a&gt;
&lt;br /&gt;&lt;br /&gt;&lt;a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85098467432&amp;amp;doi=10.1016%2Fj.ijbiomac.2020.12.063&amp;amp;partnerID=40&amp;amp;md5=3b761af76b9d8d9b7b6c8565627301df" target="_blank" rel="noreferrer noopener"&gt;https://www.scopus.com/inward/record.uri?eid=2-s2.0-85098467432&amp;amp;doi=10.1016%2fj.ijbiomac.2020.12.063&amp;amp;partnerID=40&amp;amp;md5=3b761af76b9d8d9b7b6c8565627301df&lt;/a&gt;</text>
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              <text>ISSN: 1418130; PubMed ID: 33316341; CODEN: IJBMD</text>
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              <text>Online</text>
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              <text>English</text>
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              <text>Choudhuri I., Department of Biotechnology, Panskura Banamali College, P.O.  Panskura R.S., Purba Medinipur, PIN-721152, West Bengal, India; Khanra K., Department of Biotechnology, Panskura Banamali College, P.O.  Panskura R.S., Purba Medinipur, PIN-721152, West Bengal, India; Maity P., Department of Chemistry, Sabang Sajanikanta Mahavidyalaya, Sabang, Paschim Midnapore, PIN-721166, West Bengal, India; Patra A., Department of Biotechnology, Panskura Banamali College, P.O.  Panskura R.S., Purba Medinipur, PIN-721152, West Bengal, India; Maity G.N., Department of Chemistry, Panskura Banamali College, P.O.  Panskura R.S., Purba Medinipur, PIN-721152, West Bengal, India; Pati B.R., Dept. of Microbiology, Vidyasagar University, Medinipur, PIN-721102, West Bengal, India; Nag A., Department of Life Sciences, CHRIST (Deemed to be University), PIN-560029, Bengaluru, India; Mondal S., Department of Chemistry, Panskura Banamali College, P.O.  Panskura R.S., Purba Medinipur, PIN-721152, West Bengal, India; Bhattacharyya N., Department of Biotechnology, Panskura Banamali College, P.O.  Panskura R.S., Purba Medinipur, PIN-721152, West Bengal, India</text>
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