Biotransformation of bovine tannery fleshing into utilizable product with multifunctionalities
subject: Amino acids; Delimed bovine tannery fleshing; fermented fleshing flour; lactic acid; Lactobacillus plantarum; protein hydrolysates
description: Leather industries aim at the transformation of putrescible animal raw hides and skins into a stable non-putrescible material (leather), ultimately resulting in solid wastes generation. Storage of limed bovine fleshing, a predominant proteinaceous solid waste material from pre-tanning processes is highly discouraged due to their putrescible nature. Diversely, bio-conversion of solid waste into a saleable product could be achieved by bacterial fermentation process. Lactic acid (LA) fermentation with Delimed Bovine Tannery Fleshing (DBTF) by Lactobacillus plantarum was experimented; incubation time, tapioca flour and lactose were the three prime independent variables studied. Maximum LA production in steam cooked DBTF medium was achieved with pH 6; inoculum 12.5% (v/w); lactose 8.81% (w/w) and tapioca flour 8.9% (w/w) at 96 hours of fermentation. Initial yield of about 4.56 g/L of LA (p <.05), upon statistical manipulation of the medium composition marked up to 12.36 g/L, enabling 2.7-fold increase in the LA production with DBTF as a relatively cut-rate substrate. Fermentation resulted in Fermented Fleshing Flour (FFF) with marked protein level of 76.32% and lower fibre content. Formation of protein hydrolysates and new acid groups in FFF were authenticated via Field Emission Scanning Electron Microscopic (FE-SEM) and Fourier Transform Infra-red Spectroscopic (FTIR) analyses respectively. In situ acid (LA) generation and formation of protein hydrolysates/free amino acids during LA fermentation points to the antibacterial (against Gram-negative bacteria, followed by Gram-positive bacteria) and invitro antioxidative properties with dose dependency, of FFF, favouring its possible utilization in aqua feed industries as a superior grade proteinaceous feed ingredient with inbuilt bio-functionalities.. 2020 Informa UK Limited, trading as Taylor & Francis Group.
creator: Thazeem B.; Umesh M.; Mani V.M.; Beryl G.P.; Preethi K.
source: Biocatalysis and Biotransformation, Vol-39, No. 2, pp. 81-99.
publisher: Taylor and Francis Ltd.
date: 2021-01-01
rights: Restricted Access
relation: ISSN: 10242422; CODEN: BOBOE
format: Online
language: English
type: Article
coverage: Thazeem B., Vellalar Institutions (Maruthi Nagar Campus), Thindal, India; Umesh M., Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, India; Mani V.M., Department of Biotechnology, Rathnavel Subramaniam College of Arts and Science, Coimbatore, India; Beryl G.P., Department of Microbial Biotechnology, Bharathiar University, Coimbatore, India; Preethi K., Department of Microbial Biotechnology, Bharathiar University, Coimbatore, India