Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource An ancestral genomic locus in Mycobacterium tuberculosis clinical isolates from India hints the genetic link with Mycobacterium canettii Subject The topic of the resource Ancestral strain; Genome sequencing; Mycobacterium tuberculosis; Smooth tubercle bacilli; Subtractive hybridization Description An account of the resource Background: Tuberculosis remains a worldwide public health emergency. To better understand M. tuberculosis and to identify genomic variations characteristic to the Indian clinical isolates by a low-cost method, a genomic subtractive hybridization between M. tuberculosis H37Rv and a clinical isolate from South India was performed. Results: This revealed a novel 0.4-kb subtractive fragment which was used as a handle to pull out a 4.5-kb genomic region characteristic to the clinical isolate and was absent in H37Rv. On further studies, this 4.5-kb region was found to be present in 91% of the M. tuberculosis clinical isolates screened from Kerala, a state in South India. Interestingly, this novel region has 99% identity (with 100% query coverage) with genomic regions of M. canettii. Discussion: The present study hypothesizes that this locus was present in the recent common environmental ancestor of mycobacteria, retained to the maximum extent in M. canettii and ancestral isolates of M. tuberculosis, and later deleted in other modern lineages of M. tuberculosis. Thus, this region may serve as one of the links between the pathogenic mycobacteria and the environmental species. We also propose that the Indian isolates of M. tuberculosis might be closely related to the putative progenitor M. prototuberculosis with respect to this locus. More studies on other genomic loci from different strains of M. tuberculosis are required to establish more links in this direction. 2020, Springer Nature Switzerland AG. Creator An entity primarily responsible for making the resource Sarojini S.; Mundayoor S. Source A related resource from which the described resource is derived International Microbiology, Vol-23, No. 3, pp. 397-404. Publisher An entity responsible for making the resource available Springer Date A point or period of time associated with an event in the lifecycle of the resource 2020-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1007/s10123-019-00113-0" target="_blank" rel="noreferrer noopener">https://doi.org/10.1007/s10123-019-00113-0</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85077586924&amp;doi=10.1007%2Fs10123-019-00113-0&amp;partnerID=40&amp;md5=313246f3c19fe94ecc9ad88fef0dbaaa" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85077586924&amp;doi=10.1007%2fs10123-019-00113-0&amp;partnerID=40&amp;md5=313246f3c19fe94ecc9ad88fef0dbaaa</a> Rights Information about rights held in and over the resource Restricted Access Relation A related resource ISSN: 11396709; PubMed ID: 31898033 Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Sarojini S., Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, 29, India; Mundayoor S., Rajiv Gandhi Centre for Biotechnology, (RGCB), Trivandrum, India, Inter University Centre for Biomedical Research and Super Speciality Hospital (IUCBR &amp; SSH), Kottayam, India