In vivo, in vitro and in silico screening of a potent Angiotensin Converting Enzyme (ACE) inhibitor from Trigonella foenum-graecum extract using Zebrafish as a model organism to reduce hypertension
- Title
- In vivo, in vitro and in silico screening of a potent Angiotensin Converting Enzyme (ACE) inhibitor from Trigonella foenum-graecum extract using Zebrafish as a model organism to reduce hypertension
- Creator
- Suresh T.; Sarojini S.
- Description
- The number of patients suffering from hypertension is on the rise worldwide and there is a need to explore natural products which can supplement current drugs to treat this disease. RAAS (Renin Angiotensin Aldosterone System) is one of the factors maintaining blood pressure. In the present investigation, we explored the potential of methanolic extract of fenugreek seeds in inhibiting Angiotensin Converting Enzyme (ACE), a key enzyme in the RAAS system, thereby reducing hypertension. In addition to in vivo studies conducted on zebrafish, in vitro and in silico studies were also performed to assess the inhibitory effect of the fenugreek extract on ACE. The bioactive components in Trigonella foenum-graecum revealed by GC-MS were further subjected to docking and binding studies with the receptor protein ACE. Of the various phytochemicals studied, arachidonic acid exhibited the maximum inhibitory effect on ACE. Thus, the present investigation was able to favorably screen a potent ACE inhibitor in Trigonella foenum-graecum extract which shows a potential to be used alone or supplemented with synthetic ACE inhibitors to treat high blood pressure. Further investigations are required to quantify the phytochemical for its inhibitory activity and also to understand the mechanism of inhibition of the enzyme. 2020 World Research Association. All rights reserved.
- Source
- Research Journal of Biotechnology, Vol-15, No. 2, pp. 9-16.
- Date
- 2020-01-01
- Publisher
- World Research Association
- Subject
- Angiotensin Converting Enzyme; Arachidonic acid; Hypertension; RAAS; Trigonella foenum-graecum; Zebrafish
- Coverage
- Suresh T., Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, 560029, India; Sarojini S., Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, 560029, India
- Rights
- Restricted Access
- Relation
- ISSN: 9736263
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Suresh T.; Sarojini S., “In vivo, in vitro and in silico screening of a potent Angiotensin Converting Enzyme (ACE) inhibitor from Trigonella foenum-graecum extract using Zebrafish as a model organism to reduce hypertension,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 25, 2025, https://archives.christuniversity.in/items/show/16472.