Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Articles Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Detection of carbapenem resistance genes and cephalosporin, and quinolone resistance genes along with oqxAB gene in Escherichia coli in hospital wastewater: A matter of concern Subject The topic of the resource Carbapenem resistance; Cephalosporin resistance; Hospital wastewater; Multidrug-resistant E.coli; Quinolones resistance Description An account of the resource Aims: This study was performed to detect the presence of Escherichia coli resistant to cephalosporins, carbapenems and quinolones in hospital wastewater. Methods and Results: Wastewaters from a rural (H1) and an urban (H2) hospital were tested for E.coli resistant to cephalosporins, carbapenem and quinolones. Genes coding for chromosomal and plasmid-mediated resistance and phylogenetic grouping was detected by multiplex polymerase chain reaction (PCR) and for genetic relatedness by rep-PCR. Of 190 (H1=94; H2=96) E.coli examined, 44% were resistant to both cephalosporins and quinolones and 3% to imipenem. ESBLs were detected phenotypically in 96% of the isolates, the gene blaCTX-M coding for 87% and blaTEM for 63%. Quinolone resistance was due to mutations in gyrA and parC genes in 97% and plasmid-coded aac-(6?)-Ib-cr in 89% of isolates. Only in one carbapenem-resistant E.coli, NDM-1 was detected. Nearly 67% of the isolates belonged to phylogenetic group B2. There was no genetic relatedness among the isolates. Conclusions: Hospital wastewater contains genetically diverse multidrug-resistant E.coli. Significance and Impact of the Study: This study stresses the need for efficient water treatment plants in healthcare settings as a public health measure to minimize spread of multidrug-resistant bacteria into the environment. 2014 The Society for Applied Microbiology. Creator An entity primarily responsible for making the resource Chandran S.P.; Diwan V.; Tamhankar A.J.; Joseph B.V.; Rosales-Klintz S.; Mundayoor S.; Lundborg C.S.; Macaden R. Source A related resource from which the described resource is derived Journal of Applied Microbiology, Vol-117, No. 4, pp. 984-995. Date A point or period of time associated with an event in the lifecycle of the resource 2014-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1111/jam.12591" target="_blank" rel="noreferrer noopener">https://doi.org/10.1111/jam.12591</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908243202&amp;doi=10.1111%2Fjam.12591&amp;partnerID=40&amp;md5=0d058f7506c9570f2488485b0b261426" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908243202&amp;doi=10.1111%2fjam.12591&amp;partnerID=40&amp;md5=0d058f7506c9570f2488485b0b261426</a> Rights Information about rights held in and over the resource Restricted Access Relation A related resource ISSN: 13645072; PubMed ID: 24975198; CODEN: JAMIF Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Article Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Chandran S.P., Division of Infectious Diseases, St. John's Research Institute, Bangalore, India, Department of Microbiology, St. John's Medical College, Bangalore, India; Diwan V., Department of Public Health Sciences, Global Health/IHCAR, Karolinska Institutet, Stockholm, Sweden, Department of Public Health and Environment, R.D. Gardi Medical College, Ujjain, India, International Center for Health Research, R.D. Gardi Medical College, Ujjain, India; Tamhankar A.J., Department of Public Health Sciences, Global Health/IHCAR, Karolinska Institutet, Stockholm, Sweden, Department of Environmental Medicine, Indian Initiative for Management of Antibiotic Resistance (IIMAR), R D Gardi Medical College, Ujjain, India; Joseph B.V., Departments of Biotechnology, Christ University, Bangalore, India; Rosales-Klintz S., Department of Public Health Sciences, Global Health/IHCAR, Karolinska Institutet, Stockholm, Sweden; Mundayoor S., Mycobacterial Research Group, Department of Molecular Microbiology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India; Lundborg C.S., Department of Public Health Sciences, Global Health/IHCAR, Karolinska Institutet, Stockholm, Sweden; Macaden R., Division of Infectious Diseases, St. John's Research Institute, Bangalore, India