Genetic Modification of Enzymes for Biomass Hydrolysis
Lignocellulose biomass is an economically viable and most abundant energy source. The synthesis of renewable energy-based fuel from lignocellulosic biomass is a replacement for fossil fuel. Cellulases are the biocatalysts that hydrolyze the ?-1,4-glycosidic bond in cellulose to release carbohydrate moieties that can be converted to ethanol, butanol, and other compounds. However, little enzymatic activity and product yield, and thermal stability are hurdles in the deconstruction of lignocellulose. Current progress in synthetic and omics technologies has resulted in several works in metabolic and genetic engineering that have paved the way for efficient conversion of lignocellulose to fuel in the last decades. Several works have attempted to apply genetic and metabolic engineering in the synthesis of stable and highly active cellulases at lower cost. This chapter reviews various genetic engineering technologies for enhancing cellulase synthesis and catalytic efficiency. 2024 selection and editorial matter, Reeta Rani Singhania, Anil Kumar Patel, Htor A. Ruiz, Ashok Pandey; individual chapters, the contributors.
Madhavan A.; Arun K.B.; Nair B.G.; Singhania R.R.; Raveendran S.; Awasthi M.K.; Pandey A.; Binod P.
Biomass Hydrolyzing Enzymes: Basics, Advancements, and Applications, pp. 101-111.
Madhavan A., School of Biotechnology, Amrita Vishwa Vidyapeetham, Kerala, Kollam, India; Arun K.B., Department of Life Sciences, Christ (Deemed to be University), Karnataka, Bengaluru, India; Nair B.G., School of Biotechnology, Amrita Vishwa Vidyapeetham, Kerala, Kollam, India; Singhania R.R., Institute of Aquatic Science and Technology, National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan, Centre for Energy and Environmental Sustainability, Uttar Pradesh, Lucknow, India; Raveendran S., Department of Food Technology, T K M Institute of Technology, Kerala, Kollam, India; Awasthi M.K., College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province, China; Pandey A., Centre for Energy and Environmental Sustainability, Uttar Pradesh, Lucknow, India, Centre for Innovation and Translational Research, CSIR-Indian Institute of Toxicology Research, Uttar Pradesh, Lucknow, India, Kyung Hee University, Seoul, South Korea, School of Engineering, University of Petroleum and Energy Studies, Dehradun, India; Binod P., Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Kerala, Thiruvananthapuram, India