In Vitro Production of Bioactive Compounds from Plant Cell Culture
Biosynthesis; CRISPR Cas9; Elicitation; Metabolic engineering; Plant cell culture; Plant secondary metabolites
Secondary metabolites (SMs) are bioactive compounds widely used in various industries as pharmaceutical agents and food additives and serve as precursor substances for the synthesis of commercially important products. These natural bioactive metabolites are quickly replacing chemicals as efficient coloring, flavoring, texturizing, and preservative agents. Productions of these SMs are hampered due to physiological and technological parameters. Although SMs do not have any significant role in the growth and development of the organisms where they are found, they have commercial importance. Humankind has harnessed its application in every walk of their life. In the medical field, SMs are used as antibiotics, antifungal, antiviral, metabolic inhibitors, anticancer agents, and many more. The biological and pharmacological benefits of medicinal plants are attributed to SM produced by subsidiary pathways that are highly specific to target molecules. Most pharmaceuticals are either directly or indirectly derived from plant sources. Production of SMs from field plants suffers from various limitations like seasonal production, choosing specific plant organs for specific metabolites, low yield, cost of purification, and seasonal variations. Biotechnological approaches such as plant cell, tissue, and organ cultures are the best alternative methods for commercial production. The current chapter focuses on establishment of plant cell culture system for the production of SMs, strategies to improve biomass yield and metabolite content, and biosynthetic pathways. The chapter also emphasizes elicitation strategies, application of CRISPR Cas9 in metabolite synthesis, large-scale production, and commercial aspects of SMs. The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2022.
Lakshmaiah V.V.; Banadka A.; Thirumoorthy G.; Naik P.M.; Al-Khayri J.M.; Nagella P.
Nutraceuticals Production from Plant Cell Factory, pp. 29-67.
Lakshmaiah V.V., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Banadka A., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Thirumoorthy G., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Naik P.M., Department of Botany, Karnatak University, Karnataka, Dharwad, India; Al-Khayri J.M., Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi Arabia; Nagella P., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India