Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Book Chapter Book Chapter Faculty Publications- Book Chapter Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource In Vitro Production of Bioactive Compounds from Plant Cell Culture Subject The topic of the resource Biosynthesis; CRISPR Cas9; Elicitation; Metabolic engineering; Plant cell culture; Plant secondary metabolites Description An account of the resource Secondary metabolites (SMs) are bioactive compounds widely used in various industries as pharmaceutical agents and food additives and serve as precursor substances for the synthesis of commercially important products. These natural bioactive metabolites are quickly replacing chemicals as efficient coloring, flavoring, texturizing, and preservative agents. Productions of these SMs are hampered due to physiological and technological parameters. Although SMs do not have any significant role in the growth and development of the organisms where they are found, they have commercial importance. Humankind has harnessed its application in every walk of their life. In the medical field, SMs are used as antibiotics, antifungal, antiviral, metabolic inhibitors, anticancer agents, and many more. The biological and pharmacological benefits of medicinal plants are attributed to SM produced by subsidiary pathways that are highly specific to target molecules. Most pharmaceuticals are either directly or indirectly derived from plant sources. Production of SMs from field plants suffers from various limitations like seasonal production, choosing specific plant organs for specific metabolites, low yield, cost of purification, and seasonal variations. Biotechnological approaches such as plant cell, tissue, and organ cultures are the best alternative methods for commercial production. The current chapter focuses on establishment of plant cell culture system for the production of SMs, strategies to improve biomass yield and metabolite content, and biosynthetic pathways. The chapter also emphasizes elicitation strategies, application of CRISPR Cas9 in metabolite synthesis, large-scale production, and commercial aspects of SMs. The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2022. Creator An entity primarily responsible for making the resource Lakshmaiah V.V.; Banadka A.; Thirumoorthy G.; Naik P.M.; Al-Khayri J.M.; Nagella P. Source A related resource from which the described resource is derived Nutraceuticals Production from Plant Cell Factory, pp. 29-67. Publisher An entity responsible for making the resource available Springer Nature Date A point or period of time associated with an event in the lifecycle of the resource 2022-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1007/978-981-16-8858-4_2" target="_blank" rel="noreferrer noopener">https://doi.org/10.1007/978-981-16-8858-4_2</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85160167385&amp;doi=10.1007%2F978-981-16-8858-4_2&amp;partnerID=40&amp;md5=2f887f679f0111354c6d01b47b00c077" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85160167385&amp;doi=10.1007%2f978-981-16-8858-4_2&amp;partnerID=40&amp;md5=2f887f679f0111354c6d01b47b00c077</a> Rights Information about rights held in and over the resource Restricted Access Relation A related resource ISBN: 978-981168858-4; 978-981168857-7 Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Book chapter Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Lakshmaiah V.V., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Banadka A., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Thirumoorthy G., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Naik P.M., Department of Botany, Karnatak University, Karnataka, Dharwad, India; Al-Khayri J.M., Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi Arabia; Nagella P., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India