Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Book Chapter Book Chapter Faculty Publications- Book Chapter Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Role of plant tissue culture medium components Subject The topic of the resource Carbon sources; Nanoparticles; Organic additives; Plant growth regulators; Plant tissue culture medium; Sterilization Description An account of the resource The plant tissue culture technique is one of the most acquainted techniques and has been practiced since 1902 for the development of disease-free plants with desired quality, at a larger production rate within a short span of time, irrespective of the season. Each plant has its own morphology and nutritional requirements. Moreover, the nutritional requirement for the tissues in each part of the plant varies. Thus, for the optimal growth of the desired plant, the composition and choice of the suitable media are crucial factors. The formulation of the nutrient medium for the specific tissue and the specific plant plays a vital role in the development of suitable plant tissue culture techniques. This chapter discusses the main components of the plant tissue culture media: basic components which include macroelements, microelements, nitrogen sources (including amino acids), carbon sources, organic supplements (mainly vitamins and other supplements), growth regulators like auxins, cytokinins, gibberellins, abscisic acid, ethylene, and solidifying agents. The basic components have to be incorporated at a fixed concentration. The plant growth regulators are supplemented for organogenesis and the balance between the growth regulators depends on the type of tissue culture technique adopted. The additional supplements are incorporated into the media to achieve specific responses which include activated charcoal, antioxidants, ethylene inhibitors, and complex organic additives, and antibiotics/antifungal agents are supplemented to prevent plant loss due to contamination. The chapter also emphasizes the sterilization techniques and their importance in the plant tissue culture medium. The significance of the adoption of automated media preparation and incorporation of nanoparticles in the tissue culture media is also discussed. 2022 Elsevier Inc. All rights reserved. Creator An entity primarily responsible for making the resource Sudheer W.N.; Praveen N.; Al-Khayri J.M.; Jain S.M. Source A related resource from which the described resource is derived Advances in Plant Tissue Culture: Current Developments and Future Trends, pp. 51-83. Publisher An entity responsible for making the resource available Elsevier Date A point or period of time associated with an event in the lifecycle of the resource 2022-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1016/B978-0-323-90795-8.00012-6" target="_blank" rel="noreferrer noopener">https://doi.org/10.1016/B978-0-323-90795-8.00012-6</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85139703167&amp;doi=10.1016%2FB978-0-323-90795-8.00012-6&amp;partnerID=40&amp;md5=2c0cc93762200a906ddb16f2fb0257db" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85139703167&amp;doi=10.1016%2fB978-0-323-90795-8.00012-6&amp;partnerID=40&amp;md5=2c0cc93762200a906ddb16f2fb0257db</a> Rights Information about rights held in and over the resource Restricted Access Relation A related resource ISBN: 978-032390795-8; 978-032399819-2 Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Book chapter Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Sudheer W.N., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bangalore, India; Praveen N., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bangalore, India; Al-Khayri J.M., Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi Arabia; Jain S.M., Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland