Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Reviews Review Faculty Publications- Reviews Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Biotechnological approaches for the production of camptothecin Subject The topic of the resource Camptotheca acuminata; Camptothecin; Extraction; In vitro production; NothapodytesNimmoniana; Quantification Description An account of the resource Abstract: Camptothecin (CPT), an indole alkaloid popular for its anticancer property, is considered the third most promising drug after taxol and famous alkaloids from Vinca for the treatment of cancer in humans. Camptothecin was first identified in Camptotheca acuminata followed by several other plant species and endophytic fungi. Increased harvesting driven by rising global demand is depleting the availability of elite plant genotypes, such as Camptotheca acuminata and Nothapodytes nimmoniana, crucial for producing alkaloids used in treating diseases like cancer. Conservation of these genotypes for the future is imperative. Therefore, research on different plant tissue culture techniques such as cell suspension culture, hairy roots, adventitious root culture, elicitation strategies, and endophytic fungi has been adopted for the production of CPT to meet the increasing demand without affecting the source plants existence. Currently, another strategy to increase camptothecin yield by genetic manipulation is underway. The present review discusses the plants and endophytes that are employed for camptothecin production and throws light on the plant tissue culture techniques for the regeneration of plants, callus culture, and selection of cell lines for the highest camptothecin production. The review further explains the simple, accurate, and cost-effective extraction and quantification methods. There is enormous potential for the sustainable production of CPT which could be met by culturing of suitable endophytes or plant cell or organ culture in a bioreactor scale production. Also, different gene editing tools provide opportunities for engineering the biosynthetic pathway of CPT, and the overall CPT production can be improved. Key points: Camptothecin is a naturally occurring alkaloid with potent anticancer properties, primarily known for its ability to inhibit DNA topoisomerase I. Plants and endophytes offer a potential approach for camptothecin production. Biotechnology approaches like plant tissue culture techniques enhanced camptothecin production. The Author(s) 2024. Creator An entity primarily responsible for making the resource Banadka A.; Narasimha S.W.; Dandin V.S.; Naik P.M.; Vennapusa A.R.; Melmaiee K.; Vemanna R.S.; Al-Khayri J.M.; Thiruvengadam M.; Nagella P. Source A related resource from which the described resource is derived Applied Microbiology and Biotechnology, Vol-108, No. 1 Publisher An entity responsible for making the resource available Springer Science and Business Media Deutschland GmbH Date A point or period of time associated with an event in the lifecycle of the resource 2024-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1007/s00253-024-13187-2" target="_blank" rel="noreferrer noopener">https://doi.org/10.1007/s00253-024-13187-2</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85196411618&amp;doi=10.1007%2Fs00253-024-13187-2&amp;partnerID=40&amp;md5=c46a7e4484b5ae3a71c01000b84ab7f3" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85196411618&amp;doi=10.1007%2fs00253-024-13187-2&amp;partnerID=40&amp;md5=c46a7e4484b5ae3a71c01000b84ab7f3</a> Rights Information about rights held in and over the resource All Open Access; Green Open Access; Hybrid Gold Open Access Relation A related resource ISSN: 1757598; PubMed ID: 38896329; CODEN: AMBID Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Review Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Banadka A., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bangalore, 560 029, India; Narasimha S.W., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bangalore, 560 029, India; Dandin V.S., Department of Biology, JSS College, Dharwad, India; Naik P.M., Department of Botany, Karnatak University, Karnataka, Dharwad, 580003, India; Vennapusa A.R., Department of Agriculture and Natural Resources, Delaware State University, Dover, 19901, DE, United States; Melmaiee K., Department of Agriculture and Natural Resources, Delaware State University, Dover, 19901, DE, United States; Vemanna R.S., Laboratory of Plant Functional Genomics, Regional Center for Biotechnology, Haryana, Faridabad, 121001, India; Al-Khayri J.M., Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King Faisal University, Al- Ahsa, 31982, Saudi Arabia; Thiruvengadam M., Department of Crop Science, College of Sanghuh Life Science, Konkuk University, Seoul, South Korea; Nagella P., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bangalore, 560 029, India