Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Reviews Review Faculty Publications- Reviews Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Synthetic Biology Tools for Genome and Transcriptome Engineering of Solventogenic Clostridium Subject The topic of the resource Cas; Clostridium; CRISPR; mobile intron; synthetic biology; synthetic sRNA; UTR Description An account of the resource Strains of Clostridium genus are used for production of various value-added products including fuels and chemicals. Development of any commercially viable production process requires a combination of both strain and fermentation process development strategies. The strain development in Clostridium sp. could be achieved by random mutagenesis, and targeted gene alteration methods. However, strain improvement in Clostridium sp. by targeted gene alteration method was challenging due to the lack of efficient tools for genome and transcriptome engineering in this organism. Recently, various synthetic biology tools have been developed to facilitate the strain engineering of solventogenic Clostridium. In this review, we consolidated the recent advancements in toolbox development for genome and transcriptome engineering in solventogenic Clostridium. Here we reviewed the genome-engineering tools employing mobile group II intron, pyrE alleles exchange, and CRISPR/Cas9 with their application for strain development of Clostridium sp. Next, transcriptome engineering tools such as untranslated region (UTR) engineering and synthetic sRNA techniques were also discussed in context of Clostridium strain engineering. Application of any of these discussed techniques will facilitate the metabolic engineering of clostridia for development of improved strains with respect to requisite functional attributes. This might lead to the development of an economically viable butanol production process with improved titer, yield and productivity. Copyright 2020 Kwon, Paari, Malaviya and Jang. Creator An entity primarily responsible for making the resource Kwon S.W.; Paari K.A.; Malaviya A.; Jang Y.-S. Source A related resource from which the described resource is derived Frontiers in Bioengineering and Biotechnology, Vol-8 Publisher An entity responsible for making the resource available Frontiers Media S.A. Date A point or period of time associated with an event in the lifecycle of the resource 2020-01-01 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.3389/fbioe.2020.00282" target="_blank" rel="noreferrer noopener">https://doi.org/10.3389/fbioe.2020.00282</a> <br /><br /><a href="https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084110435&amp;doi=10.3389%2Ffbioe.2020.00282&amp;partnerID=40&amp;md5=bfd6db059549743f4602eee3b8262cec" target="_blank" rel="noreferrer noopener">https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084110435&amp;doi=10.3389%2ffbioe.2020.00282&amp;partnerID=40&amp;md5=bfd6db059549743f4602eee3b8262cec</a> Rights Information about rights held in and over the resource All Open Access; Gold Open Access; Green Open Access Relation A related resource ISSN: 22964185 Format The file format, physical medium, or dimensions of the resource Online Language A language of the resource English Type The nature or genre of the resource Review Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Kwon S.W., Department of Agricultural Chemistry and Food Science Technology, Division of Applied Life Science (BK21 Plus Program), Institute of Agriculture Life Science (IALS), Gyeongsang National University, Jinju, South Korea; Paari K.A., Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, India; Malaviya A., Applied and Industrial Biotechnology Laboratory (AIBL), Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, India; Jang Y.-S., Department of Agricultural Chemistry and Food Science Technology, Division of Applied Life Science (BK21 Plus Program), Institute of Agriculture Life Science (IALS), Gyeongsang National University, Jinju, South Korea