Aloe Vera Protein Encapsulated Platinum Nanoparticles for Pain and Inflammation Therapy
Aloe vera protein; anti-inflammatory activity; carrageenan-induced paw edema model; DPPH assay; platinum nanoparticles
Platinum nanoparticles (Pt NPs) possess unique redox activity, chemical stability, and enzyme-mimetic properties, making them promising candidates for anti-inflammatory and analgesic therapies; however, protein-based green encapsulation strategies for such applications remain largely unexplored. In this study, we report the biochemically assisted synthesis of Aloe vera protein-encapsulated platinum nanoparticles (APPt NPs) using sodium borohydride as the reducing agent and freshly extracted Aloe vera proteins as both stabilizing and capping agents. The formation of Pt NPs was confirmed by a characteristic colour change and validated through physicochemical characterization. X-ray diffraction revealed a face-centred cubic crystalline structure, UVvisible spectroscopy showed absorption in the 220280 nm range due to surface plasmon resonance, and FTIR analysis confirmed the presence of protein functional groups on the nanoparticle surface. APPt NPs demonstrated significant biological efficacy. In vitro assays showed inhibition of protein denaturation (IC50 = 44.52 g/mL) and strong free-radical scavenging activity (IC50 = 10.39 g/mL). In vivo studies revealed ?60% analgesic efficacy at 50 mg kg?1 in the hot-plate model and ?50% inhibition of carrageenan-induced paw edema after 4 h, outperforming the standard drug. These results collectively highlight APPt NPs as a biocompatible, redox-active nanotherapeutic candidate for managing pain and inflammation. 2026 Wiley-VCH GmbH.
Waliaveettil, Felicia Aswathy; Edathottiyil Issac, Anila
Advanced Therapeutics;Volume;9;Issue;1;Article No.;e00477;
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https://doi.org/10.1002/adtp.202500477 https://www.scopus.com/pages/publications/105028220659?origin=resultslist
Waliaveettil F.A., Department of Physics and Electronics, CHRIST University, Karnataka, Bengaluru, India; Edathottiyil Issac A., Department of Physics and Electronics, CHRIST University, Karnataka, Bengaluru, India